Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
Mol Cell Proteomics. 2012 Dec;11(12):1853-69. doi: 10.1074/mcp.M112.022079. Epub 2012 Sep 16.
Banana and its close relative, plantain are globally important crops and there is considerable interest in optimizing their cultivation. Plantain has superior cold tolerance compared with banana and a thorough understanding of the molecular mechanisms and responses of plantain to cold stress has great potential value for developing cold tolerant banana cultivars. In this study, we used iTRAQ-based comparative proteomic analysis to investigate the temporal responses of plantain to cold stress. Plantain seedlings were exposed for 0, 6, and 24 h of cold stress at 8 °C and subsequently allowed to recover for 24 h at 28 °C. A total of 3477 plantain proteins were identified, of which 809 showed differential expression from the three treatments. The majority of differentially expressed proteins were predicted to be involved in oxidation-reduction, including oxylipin biosynthesis, whereas others were associated with photosynthesis, photorespiration, and several primary metabolic processes, such as carbohydrate metabolic process and fatty acid beta-oxidation. Western blot analysis and enzyme activity assays were performed on seven differentially expressed, cold-response candidate plantain proteins to validate the proteomics data. Similar analyses of the seven candidate proteins were performed in cold-sensitive banana to examine possible functional conservation, and to compare the results to equivalent responses between the two species. Consistent results were achieved by Western blot and enzyme activity assays, demonstrating that the quantitative proteomics data collected in this study are reliable. Our results suggest that an increase of antioxidant capacity through adapted ROS scavenging capability, reduced production of ROS, and decreased lipid peroxidation contribute to molecular mechanisms for the increased cold tolerance in plantain. To the best of our knowledge, this is the first report of a global investigation on molecular responses of plantain to cold stress by proteomic analysis.
香蕉及其近缘种芭蕉是全球重要的作物，优化其栽培具有重要意义。芭蕉具有比香蕉更强的耐寒性，深入了解芭蕉对低温胁迫的分子机制和响应，对于培育耐寒香蕉品种具有巨大的潜在价值。在本研究中，我们使用基于 iTRAQ 的比较蛋白质组学分析方法研究了芭蕉对低温胁迫的时间响应。将芭蕉幼苗在 8°C 下暴露于冷胁迫 0、6 和 24 h，随后在 28°C 下恢复 24 h。共鉴定到 3477 种芭蕉蛋白，其中 809 种蛋白在三种处理下表现出差异表达。大多数差异表达蛋白被预测参与氧化还原，包括脂氧素生物合成，而其他蛋白与光合作用、光呼吸和几种初级代谢过程相关，如碳水化合物代谢过程和脂肪酸β-氧化。对 7 种差异表达的冷响应候选芭蕉蛋白进行 Western blot 分析和酶活性测定，以验证蛋白质组学数据。在冷敏感的香蕉中对这 7 种候选蛋白进行了类似的分析，以研究可能的功能保守性，并将结果与两种物种之间的等效响应进行比较。Western blot 和酶活性测定的结果一致，表明本研究中收集的定量蛋白质组学数据是可靠的。我们的结果表明，通过适应的 ROS 清除能力增加抗氧化能力、减少 ROS 的产生和降低脂质过氧化作用，有助于增强芭蕉耐寒性的分子机制。据我们所知，这是首次通过蛋白质组学分析对芭蕉对低温胁迫的分子响应进行的全面研究。
