用于检测在兔细胞中培养的风疹病毒的抗原检测酶联免疫吸附测定法的开发与标准化。

The development and standardization of an antigen detection ELISA for rubella virus grown in rabbit cells.

作者信息

Edevåg G, Granström M

出版信息

J Biol Stand. 1986 Apr;14(2):111-9. doi: 10.1016/0092-1157(86)90029-6.

Abstract

A double antibody sandwich ELISA for the detection of rubella virus antigen was developed and standardized. Commercially available antisera were chosen in order to make the assay readily available. Antigen detection gave an excellent correlation to titers obtained by examination of cytopathogenic effect (CPE, r = 0.986). Replication of rubella virus grown in rabbit cells was identified with CPE and positive ELISA appearing within a difference of +/- one day. ELISA provided an objective detection of rubella virus which is often difficult by the reading of CPE. The method was found to be both sensitive and reproducible and facilitated work in rubella virus control involving a large number of virus titrations.

摘要

开发并标准化了一种用于检测风疹病毒抗原的双抗体夹心酶联免疫吸附测定法（ELISA）。为使该检测方法易于获得，选用了市售抗血清。抗原检测结果与通过细胞病变效应（CPE）检测获得的滴度具有极好的相关性（r = 0.986）。在兔细胞中生长的风疹病毒复制情况通过CPE鉴定，ELISA检测呈阳性，两者结果相差在±1天内出现。ELISA为风疹病毒提供了一种客观的检测方法，而通过读取CPE往往很难做到这一点。该方法被发现既灵敏又可重复，便于在涉及大量病毒滴定的风疹病毒控制工作中使用。