Park Joo-Seok, Choi Yoram, Jeong Min-Gyun, Jeong Yeong-Il, Han Ji-Hyun, Choi Hong-Kyu
Department of Applied Bioscience, Dong-A University, Busan, Republic of Korea.
Department of Molecular Genetics, Dong-A University, Busan, Republic of Korea.
Front Plant Sci. 2023 Aug 4;14:1239917. doi: 10.3389/fpls.2023.1239917. eCollection 2023.
Callus, a valuable tool in plant genetic engineering, originates from dedifferentiated cells. While transcriptional reprogramming during callus formation has been extensively studied in , our knowledge of this process in other species, such as , remains limited. To bridge this gap, our study focused on conducting a time-series transcriptome analysis of soybean callus cultured for various durations (0, 1, 7, 14, 28, and 42 days) on a callus induction medium following wounding with the attempt of identifying genes that play key roles during callus formation. As the result, we detected a total of 27,639 alterations in gene expression during callus formation, which could be categorized into eight distinct clusters. Gene ontology analysis revealed that genes associated with hormones, cell wall modification, and cell cycle underwent transcriptional reprogramming throughout callus formation. Furthermore, by scrutinizing the expression patterns of genes related to hormones, cell cycle, cell wall, and transcription factors, we discovered that auxin, cytokinin, and brassinosteroid signaling pathways activate genes involved in both root and shoot meristem development during callus formation. In summary, our transcriptome analysis provides significant insights into the molecular mechanisms governing callus formation in soybean. The information obtained from this study contributes to a deeper understanding of this intricate process and paves the way for further investigation in the field.
愈伤组织是植物基因工程中的一种重要工具，它起源于去分化细胞。虽然在[具体物种]中，愈伤组织形成过程中的转录重编程已得到广泛研究，但我们对其他物种（如[具体物种]）中这一过程的了解仍然有限。为了填补这一空白，我们的研究聚焦于对大豆愈伤组织进行时间序列转录组分析，这些愈伤组织在用创伤处理后，在愈伤组织诱导培养基上培养了不同时长（0、1、7、14、28和42天），旨在鉴定在愈伤组织形成过程中起关键作用的基因。结果，我们在愈伤组织形成过程中总共检测到27,639个基因表达变化，这些变化可分为八个不同的簇。基因本体分析表明，与激素、细胞壁修饰和细胞周期相关的基因在整个愈伤组织形成过程中经历了转录重编程。此外，通过仔细研究与激素、细胞周期、细胞壁和转录因子相关的基因的表达模式，我们发现生长素信号通路、细胞分裂素信号通路和油菜素内酯信号通路在愈伤组织形成过程中激活了参与根和茎分生组织发育的基因。总之，我们的转录组分析为大豆愈伤组织形成的分子机制提供了重要见解。本研究获得的信息有助于更深入地理解这一复杂过程，并为该领域的进一步研究铺平道路。
