采用氮选择性检测的等温气相色谱法测定血浆中纳克量的氯米帕明、氯丙嗪及其N-去甲基代谢物。

Isothermal gas chromatographic determination of nanogram amounts of chlorimipramine, chlorpromazine and their N-desmethyl metabolites in plasma using nitrogen-selective detection.

作者信息

Ninci R, Giovannini M G, Della Corte L, Sgaragli G

出版信息

J Chromatogr. 1986 Sep 5;381(2):315-22. doi: 10.1016/s0378-4347(00)83597-5.

Abstract

A gas chromatographic method using nitrogen-selective detection for the quantitative determination of nanogram amounts of chlorimipramine, chlorpromazine and their nor1 and nor2 derivatives in plasma is described. Derivatization with trifluoroacetic anhydride of nor1 and nor2 metabolites allowed the chromatographic separation of these compounds. A three-step solvent extraction procedure was performed using n-heptane containing 1% isoamyl alcohol and n-hexane and compared with a plasma clean-up procedure using C18 Sep-Pak cartridges. The two procedures were characterized by similar degrees of precision. The use of C18 Sep-Pak cartridges, however, produced a significant time and material saving over the conventional extraction method.

摘要

描述了一种采用氮选择性检测的气相色谱法，用于定量测定血浆中纳克级的氯米帕明、氯丙嗪及其去甲1和去甲2衍生物。对去甲1和去甲2代谢物用三氟乙酸酐进行衍生化处理，可实现这些化合物的色谱分离。采用含1%异戊醇的正庚烷和正己烷进行三步溶剂萃取程序，并与使用C18 Sep-Pak柱的血浆净化程序进行比较。这两种程序的精密度相似。然而，与传统萃取方法相比，使用C18 Sep-Pak柱可显著节省时间和材料。