Mozer Sahar, Abdulwahhab Intisar Ghanim, Al-Azaawie Adnan F
college of Education for Women Department of Biology, Tikrit University, Tikrit, Iraq.
college of Sciences Department of Biology Salah AI-dine, Tikrit University, Tikrit, Iraq.
J Parasit Dis. 2022 Sep;46(3):771-775. doi: 10.1007/s12639-022-01484-4. Epub 2022 May 19.
Giardiasis is one of the main diseases transmitted by food contaminated with infectious stages. It was usually spread through freshly eaten food. In this study, this parasite was diagnosed based on microscopic diagnosis of isolated samples of vegetables and fruits, as well as molecular diagnosis. The possibility of isolating parasites from vegetables and fruits and extract DNA to be used in most laboratories were verified in this study .The prevalence of were estimated on vegetables and fruits without the need to purchase a special extraction kit. .The prevalence of Giardia on fresh vegetables was estimated by using wet direct swab with Lugol's iodine dye in microscopic examination, at a rate of 4 positive samples out of 230 of all studied species, or 1.7%. DNA extraction was carried out for all samples, and 102 DNA samples were extracted from all samples, with a percentage of 44.3%. Moreover, the shown results of the nested PCR test for the 16 S rRNA gene on the obtained DNA the prevalence of Giardia lamblia at a rate of 5.9% from DNA samples isolated from vegetables and fruits.
贾第虫病是由含有感染阶段的污染食物传播的主要疾病之一。它通常通过新鲜食用的食物传播。在本研究中，这种寄生虫是基于对蔬菜和水果分离样本的显微镜诊断以及分子诊断来确诊的。本研究验证了从蔬菜和水果中分离寄生虫并提取DNA以供大多数实验室使用的可能性。在无需购买特殊提取试剂盒的情况下，对蔬菜和水果上的寄生虫感染率进行了估算。在显微镜检查中，通过使用含卢戈氏碘染料的湿直接拭子法估算新鲜蔬菜上贾第虫的感染率，在所有研究的230个物种样本中有4个阳性样本，感染率为1.7%。对所有样本进行了DNA提取，共从所有样本中提取了102个DNA样本，提取率为44.3%。此外，对所获得DNA进行的16S rRNA基因巢式PCR检测结果显示，从蔬菜和水果分离的DNA样本中，蓝氏贾第鞭毛虫的感染率为5.9%。
