Földi Dorottya, Nagy Zsófia Eszter, Belecz Nikolett, Szeredi Levente, Földi József, Kollár Anna, Tenk Miklós, Kreizinger Zsuzsa, Gyuranecz Miklós
Veterinary Medical Research Institute, Budapest, Hungary.
National Laboratory of Infectious Animal Diseases, Antimicrobial Resistance, Veterinary Public Health and Food Chain Safety, Budapest, Hungary.
Front Microbiol. 2023 Aug 4;14:1209119. doi: 10.3389/fmicb.2023.1209119. eCollection 2023.
is an emerging swine pathogen with high prevalence worldwide. The main lesions caused are arthritis and polyserositis, and the clinical manifestation of the disease may result in significant economic losses due to decreased weight gain and enhanced medical costs. We aimed to compare two challenge routes to induce infection using the same clinical isolate.
Five-week-old, Choice hybrid pigs were inoculated on 2 consecutive days by intravenous route (Group IV-IV) or by intravenous and intraperitoneal routes (Group IV-IP). Mock-infected animals were used as control (control group). After the challenge, the clinical signs were recorded for 28 days, after which the animals were euthanized. Gross pathological and histopathological examinations, PCR detection, isolation, and genotyping of the re-isolated sp. and culture of bacteria other than sp. were carried out. The ELISA test was used to detect anti- immunoglobulins in the sera of all animals.
Pericarditis and polyarthritis were observed in both challenge groups; however, the serositis was more severe in Group IV-IV. Statistically significant differences were detected between the challenged groups and the control group regarding the average daily weight gain, pathological scores, and ELISA titers. Additionally, histopathological scores in Group IV-IV differed significantly from the scores in the control group. All re-isolated strains were the same or a close genetic variant of the original challenge strain.
Our results indicate that both challenge routes are suitable for modeling the disease. However, due to the evoked more severe pathological lesions and the application being similar to the hypothesized natural route of infection in Group IV-IV, the two-dose intravenous challenge is recommended by the authors to induce serositis and arthritis associated with infection.
是一种在全球范围内高流行率的新兴猪病原体。其引起的主要病变为关节炎和多浆膜炎，由于体重增加减少和医疗成本增加，该疾病的临床表现可能导致重大经济损失。我们旨在比较两种攻毒途径，使用同一临床分离株诱导感染。
连续两天对5周龄的Choice杂交猪进行静脉注射（IV-IV组）或静脉注射和腹腔注射（IV-IP组）。将 mock 感染的动物用作对照（对照组）。攻毒后，记录28天的临床症状，之后对动物实施安乐死。进行大体病理和组织病理学检查、PCR检测、重新分离的sp.的分离和基因分型以及除sp.以外的细菌培养。使用ELISA试验检测所有动物血清中的抗免疫球蛋白。
两个攻毒组均观察到心包炎和多关节炎；然而，IV-IV组的浆膜炎更严重。在平均日增重、病理评分和ELISA滴度方面，攻毒组与对照组之间检测到统计学上的显著差异。此外，IV-IV组的组织病理学评分与对照组的评分有显著差异。所有重新分离的菌株与原始攻毒菌株相同或为其紧密的基因变体。
我们的结果表明，两种攻毒途径均适用于该疾病的建模。然而，由于IV-IV组诱发了更严重的病理病变且应用与假设的自然感染途径相似，作者建议采用两剂量静脉攻毒来诱导与感染相关的浆膜炎和关节炎。
