Yang Fan, Yang Lijun, Duan Xuecheng, Qian Yulin, Ma Huifang, Jia Xue, Huo Xinyu, Dong Wenqi, Chen Huanchun, Tan Chen
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China.
Front Vet Sci. 2025 Jun 18;12:1542992. doi: 10.3389/fvets.2025.1542992. eCollection 2025.
As a prevalent swine pathogen worldwide, (, Mhr) is associated with various diseases, including multiple serositis, pneumonia, arthritis, and otitis media. It is also linked to the porcine respiratory disease complex (PRDC).
prevalence in 2022 Chinese lung samples was assessed by species-specific PCR, followed by isolation and purification of field strains, followed by genetic characterization via multilocus sequence typing (MLST). Pathogenicity evaluation of three isolates (ZZ-1, GD-1 and AH-1) was evaluated using controlled piglet infection trials.
Mhr detection in clinical lung samples showed 31.77% prevalence. Three isolates (ZZ-1/ST166, GD-1/ST167, AH-1/ST144) were characterized by MLST. Piglet infection trials confirmed Mhr-induced polyserositis, pneumonia, and arthritis, with strain-dependent virulence variation observed.
This study confirms as a high-prevalence pathogen (31.77%) in Chinese swine herds. Animal infection models demonstrated virulence variation among different Mhr strains. These findings contribute to identifying and assessing the threats posed by different strains to pig health, guiding the development of clinical prevention and control strategies.
作为一种在全球范围内普遍存在的猪病原体，（，Mhr）与多种疾病相关，包括多发性浆膜炎、肺炎、关节炎和中耳炎。它还与猪呼吸道疾病综合征（PRDC）有关。
通过种特异性PCR评估2022年中国肺部样本中的流行情况，随后分离和纯化田间菌株，然后通过多位点序列分型（MLST）进行基因特征分析。使用对照仔猪感染试验评估三株分离株（ZZ-1、GD-1和AH-1）的致病性。
临床肺部样本中的Mhr检测显示流行率为31.77%。通过MLST对三株分离株（ZZ-1/ST166、GD-1/ST167、AH-1/ST144）进行了特征分析。仔猪感染试验证实Mhr可引起多发性浆膜炎、肺炎和关节炎，并观察到菌株依赖性毒力变异。
本研究证实Mhr是中国猪群中一种高流行率的病原体（31.77%）。动物感染模型证明了不同Mhr菌株之间的毒力差异。这些发现有助于识别和评估不同菌株对猪健康构成的威胁，指导临床预防和控制策略的制定。
