Setor de Patologia Veterinária, Departamento de Patologia Clínica Veterinária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Laboratório de Virologia Veterinária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
J Med Primatol. 2023 Dec;52(6):392-399. doi: 10.1111/jmp.12670. Epub 2023 Aug 21.
Simplexvirus humanalpha1 (HuAHV-1) are common anthropozoonosis reported in marmosets but rare in howler monkeys (Alouatta sp.).
Necropsy of two brown-howler monkeys (A. caraya) and one red-howler monkey (A. guariba clamitans) from different zoo collections were performed. Fragments of all organs were examined through microscopy. Samples were submitted to IHC for Simplexvirus humanalpha 2 (HuAHV-2) [sin. Herpesvirus simplex type 2] and PCR.
Grossly, only the A. guariba showed liver lesions characterized by multifocal, pinpoint white areas corresponding microscopically as random necrotizing herpetic hepatitis and ulcerative glossitis. Both A. caraya showed necrotizing meningoencephalitis with Cowdry A-type body inclusions within neurons and astrocytes. Immunolabeling for HuAHV-1/2 was observed in the tongue, liver, and brain. HuAHV-1 was confirmed in all samples by PCR, Sanger sequencing, and phylogenetic analyses.
Necrotizing meningoencephalitis was appreciated in 2/3 of animals, and it is associated with neurologic signs. Along with ulcerative glossitis, a hallmark lesion in marmosets, it was present in one animal. Regarding herpetic hepatitis, it is not frequent in monkeys and occurs mainly in immunocompromised animals. HuAHV-1 infection was confirmed corroborating with a human source. This is the second report on captive black-howler monkeys and the first gross, histologic, immunohistochemical, and molecular description of herpetic hepatitis and ulcerative glossitis in red-howler monkeys (A. guariba).
simplexvirus humanalpha1（HuAHV-1）是在狨猴中常见的人畜共患病，但在吼猴（Alouatta sp.）中罕见。
对来自不同动物园收藏的两只棕吼猴（A. caraya）和一只红吼猴（A. guariba clamitans）进行尸检。通过显微镜检查所有器官的碎片。将样本提交给 IHC 进行 Simplexvirus humanalpha 2（HuAHV-2）[sin. Herpesvirus simplex type 2]和 PCR 检测。
大体上，只有 A. guariba 显示出肝脏病变，其特征是多灶性、针尖状白色区域，显微镜下对应随机坏死性疱疹性肝炎和溃疡性舌炎。A. caraya 均显示出坏死性脑膜脑炎，神经元和星形胶质细胞内有 Cowdry A 型包涵体。在舌、肝和脑内观察到 HuAHV-1/2 的免疫标记。PCR、Sanger 测序和系统发育分析证实了 HuAHV-1 在所有样本中的存在。
在 3 只动物中有 2 只出现坏死性脑膜脑炎，与神经系统症状相关。与在狨猴中常见的溃疡性舌炎一起，在一只动物中存在。关于疱疹性肝炎，在猴子中并不常见，主要发生在免疫功能低下的动物中。HuAHV-1 感染得到了证实，与人类来源相符。这是关于圈养黑吼猴的第二份报告，也是首次对红吼猴（A. guariba）的疱疹性肝炎和溃疡性舌炎进行大体、组织学、免疫组织化学和分子描述。
