Souza Fabiana, Alfieri Amauri A, Alfieri Alice F, Lorenzetti Elis, Headley Selwyn A, Passos Fernando, Silvestre Thiago, Zago Luciana, Mottin Viviane, Montiani-Ferreira Fabiano, Lange Rogério, Svoboda Walfrido, Gomes Eliane
Graduate Program in Pharmaceutical Sciences, Universidade Federal do Paraná, Curitiba, Brazil.
J Med Primatol. 2012 Oct;41(5):304-8. doi: 10.1111/j.1600-0684.2012.00557.x. Epub 2012 Aug 13.
This study evaluated the presence of rotavirus groups A, B, and C (RV-A, RV-B, and RV-C), sapovirus (SaV), and norovirus (NoV) in asymptomatic non-human primates (NHP).
Fecal samples were collected from 19 recently captured (Red-howler, Alouatta guariba clamitans, n = 18; Howler, Alouatta caraya, n = 1) and 43 free-ranging NHP (Marmosets, Callithrix spp., Callithrix penicillata, n = 30; Black-faced lion tamarin, Leontopithecus caissara, n = 12, Red-howler, Alouatta guariba clamitans, n = 1) that were maintained in southern Brazil without manifestation of diarrhea. Screening was performed by a combination of silver-stained polyacrylamide gel electrophoresis (ss-PAGE) and RT-PCR analyses.
All samples were negative for RV-A, RV-B, RV-C, SaV, and NoV by both assays.
The negative results obtained might be due to the absence of clinical manifestations of disease in the population of NHP evaluated.
本研究评估了无症状非人灵长类动物（NHP）中A、B和C组轮状病毒（RV-A、RV-B和RV-C）、札幌病毒（SaV）和诺如病毒（NoV）的存在情况。
从19只新捕获的（红吼猴，Alouatta guariba clamitans，n = 18；吼猴，Alouatta caraya，n = 1）和43只散养的NHP（狨猴，Callithrix spp.，Callithrix penicillata，n = 30；黑面狮狨，Leontopithecus caissara，n = 12，红吼猴，Alouatta guariba clamitans，n = 1）收集粪便样本，这些动物饲养在巴西南部，无腹泻表现。通过银染聚丙烯酰胺凝胶电泳（ss-PAGE）和逆转录-聚合酶链反应（RT-PCR）分析相结合的方法进行筛查。
两种检测方法均显示所有样本的RV-A、RV-B、RV-C、SaV和NoV均为阴性。
所获得的阴性结果可能是由于所评估的NHP群体中无疾病临床表现所致。
