Division of Microbiology and Biotechnology, Yenepoya Research Centre, Yenepoya (Deemed to be University), University Road, Deralakatte, Mangaluru, 575018, India.
Department of Pediatrics, Yenepoya Medical College Hospital, Yenepoya (Deemed to be University), Deralakatte, Mangaluru, 575018, India.
Arch Microbiol. 2023 Aug 21;205(9):312. doi: 10.1007/s00203-023-03653-2.
Neonatal sepsis is a severe bacterial infection that can lead to life-threatening complications in newborns. Pseudomonas extremorientalis is a Gram-negative bacterium and these Gram-negative organisms have been identified as a major cause of neonatal sepsis. The virulence factors produced by this bacterium play a crucial role in its pathogenicity. Therefore, targeting these virulence factors could be a potential strategy to treat neonatal sepsis caused by P. extremorientalis. In this study, we investigated the efficacy of 3-(bromoacetyl) coumarin (3-BC) in reducing the virulence factors of P. extremorientalis strains isolated from neonatal sepsis. Our results showed that 3-BC effectively reduced the production of various virulence factors, including protease, elastase, siderophore, and exopolysaccharide in these strains. Furthermore, at a concentration of 125 µg/ml, 3-BC also inhibited the biofilm formation ability of these strains in combination with ciprofloxacin. It was discovered that 3-BC was functionally effective in protecting C. elegans against bacterial infection. Moreover, the in vitro and in vivo outcomes were strongly correlated with docking studies of various activator proteins. Overall, our findings suggest that 3-BC could be a potential therapeutic agent for the treatment of neonatal sepsis caused by P. extremorientalis. Further studies are needed to explore the mechanism of action of 3-BC and its potential use in clinical settings.
新生儿败血症是一种严重的细菌感染，如果不及时治疗，可能会导致新生儿出现危及生命的并发症。极端东方假单胞菌是一种革兰氏阴性菌，这些革兰氏阴性菌已被确定为新生儿败血症的主要原因。该细菌产生的毒力因子在其致病性中起着至关重要的作用。因此，针对这些毒力因子可能是治疗由极端东方假单胞菌引起的新生儿败血症的一种潜在策略。在这项研究中，我们研究了 3-(溴乙酰基)香豆素（3-BC）在降低从新生儿败血症分离的极端东方假单胞菌菌株的毒力因子方面的疗效。我们的结果表明，3-BC 能有效降低这些菌株产生的各种毒力因子，包括蛋白酶、弹性蛋白酶、铁载体和胞外多糖。此外，在 125μg/ml 的浓度下，3-BC 还与环丙沙星联合抑制了这些菌株的生物膜形成能力。发现 3-BC 在功能上有效保护秀丽隐杆线虫免受细菌感染。此外，体外和体内结果与各种激活蛋白的对接研究密切相关。总的来说，我们的研究结果表明，3-BC 可能是治疗由极端东方假单胞菌引起的新生儿败血症的一种潜在治疗剂。需要进一步研究来探索 3-BC 的作用机制及其在临床环境中的潜在用途。
