National Engineering Research Center of Industrial Wastewater Detoxication and Resource Recovery, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China; State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China.
National Engineering Research Center of Industrial Wastewater Detoxication and Resource Recovery, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China; State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China.
Bioresour Technol. 2023 Nov;387:129700. doi: 10.1016/j.biortech.2023.129700. Epub 2023 Aug 19.
The use of indole-3-acid (IAA) as an additive aided in achieving the objectives of reducing sludge extract toxicity, increasing Tetradesmus obliquus biomass yield, and enhancing extracellular polysaccharide production. Proteomics analysis can unveil the microalgae's response mechanism to sludge toxicity stress. With 10 M IAA addition, microalgae biomass reached 3.426 ± 0.067 g/L. Sludge extract demonstrated 78.3 ± 3.2% total organic carbon removal and 72.2 ± 2.1% toxicity removal. Extracellular polysaccharides and proteins witnessed 2.08 and 1.76-fold increments, respectively. Proteomic analysis indicated that Tetradesmus obliquus directed carbon sources towards glycogen accumulation and amino acid synthesis, regulating pathways associated with carbon metabolism (glycolysis, TCA cycle, and amino acid metabolism) to adapt to the stressful environment. These findings lay the groundwork for future waste sludge treatment and offer novel insights into microalgae cultivation and extracellular polysaccharide enrichment in sludge.
吲哚-3-乙酸（IAA）的使用有助于实现减少污泥提取物毒性、提高斜生栅藻生物质产量和提高胞外多糖产量的目标。蛋白质组学分析可以揭示微藻对污泥毒性胁迫的响应机制。添加 10 M IAA 后，微藻生物量达到 3.426±0.067 g/L。污泥提取物的总有机碳去除率为 78.3±3.2%，毒性去除率为 72.2±2.1%。胞外多糖和蛋白质分别增加了 2.08 倍和 1.76 倍。蛋白质组学分析表明，斜生栅藻将碳源导向糖原积累和氨基酸合成，调节与碳代谢（糖酵解、三羧酸循环和氨基酸代谢）相关的途径以适应胁迫环境。这些发现为未来的废污泥处理奠定了基础，并为微藻培养和污泥中胞外多糖的富集提供了新的见解。
