National Engineering Research Center of Industrial Wastewater Detoxication and Resource Recovery, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai, 200237, China; State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai, 200237, China.
National Engineering Research Center of Industrial Wastewater Detoxication and Resource Recovery, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai, 200237, China; State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai, 200237, China.
Environ Res. 2024 Dec 15;263(Pt 1):120079. doi: 10.1016/j.envres.2024.120079. Epub 2024 Sep 27.
The utilization of residual sludge by microalgae represents an environmentally sustainable method for resource recovery. In this study, Tetradesmus obliquus was cultured in hydrolysate derived from toxic sludge. Under symbiotic conditions with bacteria, Tetradesmus obliquus demonstrated enhanced toxin degradation capability and biomass accumulation, which exhibited a 1.39-fold increase in algal cell density, a 1.50-fold increase in Rubisco activity, and a total protein content of 341.83 ± 6.99 mg/L on the 30th day of cultivation. Metabolic utilization of substances in the hydrolysate by microalgae led to a toxicity removal rate of up to 60.43% by day 10. Phenylalanine showed the most significant increase among essential amino acids, and transcriptomic profiling identified genes (gene_16399, gene_16602) involved in phenylalanine enrichment. Macrotranscriptomics showed that bacteria upregulated the TCS system and tryptophan metabolism, supplying microalgae with more CO and IAA, which enhanced amino acid enrichment. This study established a non-toxic and biomass-accumulating bacterial-algal co-cultivation system.
利用微藻处理剩余污泥是一种环境可持续的资源回收方法。在这项研究中，斜生栅藻在源自有毒污泥的水解产物中进行培养。在与细菌共生的条件下，斜生栅藻表现出增强的毒素降解能力和生物量积累，在培养的第 30 天，藻细胞密度增加了 1.39 倍，Rubisco 活性增加了 1.50 倍，总蛋白含量达到 341.83 ± 6.99mg/L。微藻对水解产物中物质的代谢利用导致毒性去除率在第 10 天达到了 60.43%。必需氨基酸中苯丙氨酸的增加最为显著，转录组分析鉴定了参与苯丙氨酸富集的基因（基因_16399、基因_16602）。宏转录组学表明，细菌上调了 TCS 系统和色氨酸代谢，为微藻提供了更多的 CO 和 IAA，从而增强了氨基酸的富集。本研究建立了一种无毒且能积累生物质的细菌-藻类共培养系统。
