Fermentation Regulation Research Group, World Institute of Kimchi, Gwangju, Republic of Korea.
Food Analysis Research Center, Korea Food Research Institute, Wanju, Republic of Korea.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2023 Sep;40(9):1275-1284. doi: 10.1080/19440049.2023.2245055. Epub 2023 Aug 22.
Ochratoxin A (OTA) is a stable toxin produced by fungal strains of and . It is commonly found in a variety of food products, including dried fruit, coffee, and spices, raising concerns about their safety. This study was aimed to quantify OTA levels in different food products using HPLC with fluorescence detection. The pre-treatment process was optimised by employing immunoaffinity columns with Tween 20 to effectively remove interfering substances. An analytical method was developed, validated, and applied for OTA analysis in dried fruit, spices, and coffee samples. The validation procedure included determining detection and quantification limits, linearity, precision, and accuracy, as per the criteria specified by AOAC International. The validated method was successfully applied for OTA analysis in the selected food samples. Furthermore, health risk assessment was conducted based on the average intake and body weight of the Korean population. From the results, concentrations of OTA in the samples were found to be very low and therefore concluded not to pose significant threats to consumer health.
赭曲霉毒素 A（OTA）是一种由 和 真菌菌株产生的稳定毒素。它通常存在于各种食品中，包括干果、咖啡和香料，引起了对其安全性的关注。本研究旨在使用带有荧光检测的 HPLC 定量测定不同食品中的 OTA 水平。通过使用含吐温 20 的免疫亲和柱优化预处理过程，有效地去除了干扰物质。开发、验证并应用了一种分析方法，用于测定干果、香料和咖啡样品中的 OTA。验证程序包括根据 AOAC 国际规定的标准确定检测限和定量限、线性、精密度和准确度。该验证方法成功应用于选定食品样品中的 OTA 分析。此外，还根据韩国人口的平均摄入量和体重进行了健康风险评估。结果表明，样品中的 OTA 浓度非常低，因此不会对消费者健康构成重大威胁。
