School of Biological Science and Technology, University of Jinan, Jinan 250022, China; and School of Pharmaceutical Sciences, Shandong University of Traditional Chinese Medicine, Jinan 250355, PR China.
School of Biological Science and Technology, University of Jinan, Jinan 250022, China.
Funct Plant Biol. 2023 Oct;50(10):808-820. doi: 10.1071/FP23033.
The flower buds of Lonicera japonica are widely used for its high medicinal value. It is reported that the accumulation of phenylpropanoids in the buds of L. japonica is affected by the stage at which it is harvested. However, the changes of active components and the underlying mechanisms in flower buds at different harvesting stages have not been reported. Integrative analyses of transcriptomics and metabolomics was used to explore the underlying mechanism of harvesting stages (green bud, GB; and white bud, WB) on the phenylpropanoids metabolites accumulation in L. japonica . The result showed that 3735 differentially expressed genes were identified, and the genes related to glycolysis/gluconeogenesis and phenylalanine biosynthesis pathway were significantly upregulated in GB stage. A total of 510 differential metabolites were identified in GB stage. Among them, 14 phenylpropanoids were changed during the GB and WB, seven of which increased in GB, including caffeic acid, sauchinone, coniferin, secoisolariciresinol diglucoside, scopolin, methyl cinnamate, chlorogenic acid, 7-hydroxycoumarin, while others such as sibiricose A6, coumarin, eleutheroside E decreased. Further correlation analysis showed that the unigenes for CSE, CAD, bg1, ADH, ALDH, DLAT and ENO significantly correlated with the 10 phenylpropanoid. The above results would provide basic data for the selection of harvesting stages in the production of L. japonica .
金银花的花蕾因其药用价值高而被广泛应用。据报道，金银花花蕾中苯丙素类化合物的积累受其收获阶段的影响。然而，不同收获阶段花蕾中活性成分的变化及其潜在机制尚未报道。本研究采用转录组学和代谢组学综合分析方法，探讨了不同收获阶段（绿蕾期、GB 和白蕾期、WB）对金银花中苯丙素类代谢物积累的潜在机制。结果表明，共鉴定到 3735 个差异表达基因，其中与糖酵解/糖异生和苯丙氨酸生物合成途径相关的基因在 GB 期显著上调。在 GB 期共鉴定到 510 个差异代谢物。其中，14 种苯丙素类物质在 GB 和 WB 期间发生变化，其中 7 种在 GB 期增加，包括咖啡酸、蛇床子素、松柏苷、松脂醇二葡萄糖苷、莨菪亭、肉桂酸、绿原酸、7-羟基香豆素，而其他物质如西伯利亚酮 A6、香豆素、刺五加苷 E 则减少。进一步的相关性分析表明，CSE、CAD、bg1、ADH、ALDH、DLAT 和 ENO 的 unigenes 与 10 种苯丙素类物质显著相关。上述结果为金银花的生产中选择收获阶段提供了基础数据。
