gen. nov., sp. nov., isolated from activated sludge.

A bacterial strain designated as LB-31 was isolated from activated sludge. Strain LB-31 was Gram-stain-negative, non-spore-forming and rod-shaped with a single polar flagellum. The isolate grew at 15-37 °C, pH 5.5-8.5 and 0.5-8.5 % (w/v) NaCl. Genome sequencing revealed a genome size of 3.55 Mb, a G+C content of 61.1 mol% and 3222 protein-coding genes. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain LB-31 formed a phyletic lineage that was distinct from the three known genus within the family . The 16S rRNA gene sequence similarity of strain LB-31 to all recognized bacterial species was no more than 94 %. Average nucleotide identity and digital DNA-DNA hybridization values between strain LB-31 and its phylogenetic neighbours were below 72 and 22 %, respectively. The average amino acid identity values for strain LB-31 and its closed phylogenetic neighbours and were below 63%, supporting that strain LB-31 was a member of a novel genus. The predominant respiratory quinone is Q-10, the fatty acid profile predominantly consisted of iso-C 9, iso-C, iso-C and summed feature 3 (comprising iso-C2-OH/C 7), and the major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The combined genotypic and phenotypic data show that strain LB-31 represents a novel species of a novel genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is LB-31 (=GDMCC 1.3625=KCTC 92687).