Key Laboratory of Agricultural Microbiomics and Precision Application (MARA), Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiome (MARA), State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, PR China.
Int J Syst Evol Microbiol. 2023 Jun;73(6). doi: 10.1099/ijsem.0.005953.
A Gram-stain-negative, facultatively anaerobic, motile and rod-shaped bacterium, designated as 6D33, was isolated from mangrove soil. Growth was found to occur at 15-32 °C (optimum, 28 °C), at pH 6-9 (optimum, pH 7) and in 0-3 % NaCl (optimum, 1 %, w/v). The results of 16S rRNA gene-based analysis showed that strain 6D33 belonged to the family , sharing 93.1-94.4 % identity with its close neighbours within the genus . The phylogenomic results indicated that strain 6D33 formed an independent branch distinct from type strains of the genus . The overall genome relatedness indices of digital DNA-DNA hybridization, average nucleotide identity and amino acid identity values showed that strain 6D33 represents a novel species of a novel genus. The results of chemotaxonomic characterization indicated that the major cellular fatty acids of strain 6D33 were summed feature 9 (C 10-methyl and/or iso-C 9), summed feature 3 (C 6 and/or C 7) and iso-C; the polar lipids comprised diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminolipid and three unidentified lipids; the only respiratory quinone was ubiquinone-10. The genomic size and DNA G+C contents were 3.59 Mbp and 60.84 mol%, respectively. The 16S rRNA gene sequence reads abundance profiles revealed that the rare taxon is prevalent in marine environments, especially in sediments. Genome-scale metabolic reconstruction of strain 6D33 revealed a heterotrophic lifestyle and many pathways responsible for the degradation of aromatic compounds, suggesting application potential in aromatic hydrocarbon removal. Based on its genotypic and phenotypic characteristics, strain 6D33 is concluded to represent a novel species of the novel genus in the family , for which the name gen. nov. sp. nov. is proposed. The type strain of the type species is 6D33 (=GDMCC 1.1959=KCTC 82335).
一株革兰氏阴性、兼性厌氧、运动和杆状的细菌，被命名为 6D33，从红树林土壤中分离得到。该菌在 15-32°C（最佳温度 28°C）、pH 值 6-9（最佳 pH 值 7）和 0-3%NaCl（最佳 1%，w/v）的条件下生长。基于 16S rRNA 基因的分析结果表明，菌株 6D33 属于 科，与属内近缘种的同源性为 93.1-94.4%。系统基因组学分析结果表明，菌株 6D33 形成了一个与属内模式种不同的独立分支。数字 DNA-DNA 杂交、平均核苷酸同一性和氨基酸同一性值的全基因组相关性指数表明，菌株 6D33 代表了一个新属的新种。化学分类特征的结果表明，菌株 6D33 的主要细胞脂肪酸为总和特征 9（C 10-甲基和/或异 C 9）、总和特征 3（C 6 和/或 C 7）和异 C；极性脂包括双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、一种未鉴定的胺脂和三种未鉴定的脂；唯一的呼吸醌是泛醌-10。基因组大小和 DNA G+C 含量分别为 3.59 Mbp 和 60.84 mol%。16S rRNA 基因序列读长丰度图谱显示，稀有分类群在海洋环境中普遍存在，特别是在沉积物中。菌株 6D33 的基因组规模代谢重建表明其为异养生活方式，有许多途径负责芳香族化合物的降解，这表明其在芳香烃去除方面具有应用潜力。基于其基因型和表型特征，菌株 6D33 被认为代表了一个新属的新种，该属被命名为 属，新种命名为 。模式种的模式株为 6D33（=GDMCC 1.1959=KCTC 82335）。
