State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, P. R. China.
National Engineering Laboratory for AIDS Vaccine, School of Life Science, Jilin University, Changchun 130012, P. R. China.
Nano Lett. 2023 Sep 13;23(17):8363-8369. doi: 10.1021/acs.nanolett.3c02764. Epub 2023 Aug 23.
The crosstalk between mitochondria and endoplasmic reticula plays a crucial role in apoptotic pathways in which reactive oxygen species (ROS) produced by microsomal monooxygenase (MMO) are believed to accelerate cytochrome c release. Herein, we successfully demonstrate the potential of surface-enhanced resonance Raman spectroscopy (SERRS) for monitoring MMO-derived ROS formation and ROS-mediated cytochrome c release. Silver nanoparticles coated with nickel shells are used as both Raman signal enhancers and electron donors for cytochrome c. SERRS of cytochrome c is found to be sensitive to ROS, allowing for in situ probing of ROS formation with a cell death inducer. Label-free evaluation of ROS-induced apoptosis is achieved by SERRS-based monitoring of cytochrome c release in living cells. This study verifies the capability of SERRS for label-free, in situ, and real-time monitoring of the mitochondria-endoplasmic reticulum crosstalk in apoptosis and provides a novel strategy for the rational design and screening of ROS-inducing drugs for cancer treatment.
线粒体和内质网之间的串扰在细胞凋亡途径中起着关键作用，其中微粒体单加氧酶 (MMO) 产生的活性氧 (ROS) 被认为可以加速细胞色素 c 的释放。在此，我们成功地证明了表面增强共振拉曼光谱 (SERRS) 用于监测 MMO 衍生的 ROS 形成和 ROS 介导的细胞色素 c 释放的潜力。用镍壳涂覆的银纳米粒子既可用作拉曼信号增强剂，也可用作细胞色素 c 的电子供体。发现细胞色素 c 的 SERRS 对 ROS 敏感，允许用细胞死亡诱导剂原位探测 ROS 的形成。通过基于 SERRS 的对活细胞中细胞色素 c 释放的监测，实现了对 ROS 诱导的细胞凋亡的无标记评估。这项研究验证了 SERRS 用于无标记、原位和实时监测细胞凋亡中线粒体-内质网串扰的能力，并为合理设计和筛选用于癌症治疗的 ROS 诱导药物提供了一种新策略。
