School of Pharmacy, Shenyang Pharmaceutical University, No. 26 Huatuo Rd, High & New Tech Development Zone, 117004, Benxi, Liaoning Province, People's Republic of China.
Mikrochim Acta. 2023 Aug 23;190(9):365. doi: 10.1007/s00604-023-05945-2.
A novel pH-responsive magnetic graphene oxide composite (MGO@PEI-BA) is proposed for the first time as an adsorbent for the rapid capture and detection of nucleosides (cytidine, uridine, guanosine, and adenosine). The morphology, structure, and magnetic properties of the composite were evaluated using various characterization techniques. The results indicated that the composite was successfully fabricated. A series of parameters that affect extraction and elution were optimized through one-factor-at-a-time and Box-Behnken design of response surface methodology (BBD-RSM). The unique layered structures and easily accessible active sites of the composite facilitated molecular transport, resulting in instantaneous equilibrium of nucleosides adsorption within 5 min. Based on this study, a magnetic dispersive micro-solid-phase extraction (MD-μ-SPE) method assisted by the MGO@PEI-BA was developed in combination with UHPLC-UV analysis for the determination of nucleosides in rat urine. Under the optimum conditions, a wide linear range (10-2000 ng mL), good linearity (r > 0.99), low detection limits (1-3 ng mL), low relative standard deviations (RSDs ≤ 3.9%), and satisfactory recoveries (82.7-96.3%) were achieved. These results demonstrate that the MGO@PEI-BA is an excellent adsorbent for extracting nucleosides from biological samples.
一种新型的 pH 响应型磁性氧化石墨烯复合材料（MGO@PEI-BA）被首次提出作为一种吸附剂，用于快速捕获和检测核苷（胞苷、尿苷、鸟苷和腺苷）。使用各种表征技术评估了复合材料的形态、结构和磁性。结果表明复合材料成功制备。通过单因素实验和响应面法（BBD-RSM）的 Box-Behnken 设计优化了一系列影响提取和洗脱的参数。该复合材料独特的层状结构和易于接近的活性位点促进了分子的传输，从而使核苷的吸附在 5 分钟内达到瞬时平衡。基于这项研究，开发了一种由 MGO@PEI-BA 辅助的磁性分散微固相萃取（MD-μ-SPE）方法，结合 UHPLC-UV 分析用于测定大鼠尿液中的核苷。在最佳条件下，获得了较宽的线性范围（10-2000 ng mL）、良好的线性（r>0.99）、低检测限（1-3 ng mL）、低相对标准偏差（RSDs≤3.9%）和令人满意的回收率（82.7-96.3%）。这些结果表明，MGO@PEI-BA 是一种从生物样品中提取核苷的优异吸附剂。
