Barratt C L, Cohen J
J Reprod Fertil. 1986 Sep;78(1):1-10. doi: 10.1530/jrf.0.0780001.
The progression of 3H-labelled spermatozoa (thymidine or arginine) was followed through the tracts of unilaterally vasectomized, bilaterally vasectomized, oligozoospermic (t6/tw5) and normal mice; the regional lymph nodes were also investigated. The same rate of sperm production and transport was found in normal and in vasectomized tracts, down to the corpus epididymidis; there was some delay in spermatozoa entering the cauda in the vasectomized tracts. In the mouse, therefore, vasectomy does not affect the rates of sperm production or transport until just before the blockage in the swollen cauda epididymidis. Radioactivity appeared in the caudal and 'para-aortic' lymph nodes as the radioactive spermatozoa passed from the corpus, showing that this is one route of disposal of spermatozoa, or of sperm products, after vasectomy. Naturally oligozoospermic and normal mice gave similar results; again the caudal, iliac and renal lymph nodes received radioactive spermatozoa/sperm products. Some loss of (by definition) superfluous spermatozoa in the normal male tract therefore occurs naturally by this route, and we suggest that vasectomy further exploits this physiological pathway. This would account for the finding that many males do not make antisperm antibodies after vasectomy, just as normal males do not, even though their lymph nodes normally receive spermatozoa/sperm products.
追踪3H标记的精子（胸腺嘧啶核苷或精氨酸）在单侧输精管结扎、双侧输精管结扎、少精子症（t6/tw5）和正常小鼠的生殖道中的进程；同时也对区域淋巴结进行了研究。在正常和输精管结扎的生殖道中，直至附睾体，精子产生和运输的速率相同；在输精管结扎的生殖道中，精子进入附睾尾存在一定延迟。因此，在小鼠中，输精管结扎在肿胀的附睾尾阻塞之前不会影响精子产生或运输的速率。当放射性精子从附睾体通过时，放射性出现在附睾尾和“主动脉旁”淋巴结，这表明这是输精管结扎后精子或精子产物的一种处置途径。自然少精子症小鼠和正常小鼠给出了相似的结果；同样，附睾尾、髂骨和肾淋巴结接收了放射性精子/精子产物。因此，正常雄性生殖道中（从定义上讲）多余的精子会通过这条途径自然损失一部分，我们认为输精管结扎进一步利用了这一生理途径。这可以解释为什么许多男性在输精管结扎后不会产生抗精子抗体，就像正常男性一样，即使他们的淋巴结通常会接收精子/精子产物。
