Grossman H B, Wedemeyer G, Ren L, Wilson G N, Cox B
J Urol. 1986 Oct;136(4):953-9. doi: 10.1016/s0022-5347(17)45139-1.
From January, 1981 through June, 1982 specimens from 21 patients with bladder (urothelial) cancer were placed in tissue culture, and one long term cell line was established (5%). From July, 1982 through February, 1984, using an improved culture medium, seven long term cell cultures were established from 21 patients (33%). In addition, one long term culture from a patient with a bladder melanoma was established using the standard culture medium. The nine cell cultures were derived from the following types of tumors: transitional cell carcinoma (6), adenocarcinoma (1), squamous cell carcinoma (1) and melanoma (1). All of the cell lines have produced tumors in athymic nude mice except for one transitional cell carcinoma. All of the cultures demonstrate aneuploidy. Homogeneously staining regions have been seen in some cell cultures. A common marker chromosome has not been identified.
从1981年1月至1982年6月，将21例膀胱（尿路上皮）癌患者的标本进行组织培养，建立了1个长期细胞系（5%）。从1982年7月至1984年2月，使用改良培养基，从21例患者中建立了7个长期细胞培养物（33%）。此外，使用标准培养基从1例膀胱黑色素瘤患者中建立了1个长期培养物。这9个细胞培养物来源于以下类型的肿瘤：移行细胞癌（6例）、腺癌（1例）、鳞状细胞癌（1例）和黑色素瘤（1例）。除1例移行细胞癌外，所有细胞系均在无胸腺裸鼠中产生了肿瘤。所有培养物均显示非整倍体。在一些细胞培养物中可见均匀染色区。尚未鉴定出共同的标记染色体。
