School of Materials Science and Engineering, Chang' an University, Xi'an, 710062, Shaanxi, China.
Shanghat University of Medicine Health Sciences Affiliated Zhoupu Hospital, Shanghai, 201300, China.
Mikrochim Acta. 2023 Aug 24;190(9):366. doi: 10.1007/s00604-023-05949-y.
The excessive utilization of antibiotics has led to significant water contamination and posed severe threats to human well-being. Consequently, the pressing imperative to identify antibiotics in the environment arises. In this study, we have successfully synthesized a hollow PCN-222 MOF distinguished by its substantial surface area and abundant functional groups, particularly the porphyrin cores. To augment the electrical conductivity of the hollow PCN-222 (HPCN-222), gold (Au) particles were incorporated within the porphyrin core using a fundamental hydrothermal method. This modification facilitated the effective immobilization of aptamer strands through π-π stacking and electrostatic interactions. As a result, the Au@HPCN-222 composite demonstrated exceptional efficacy as a substrate for immobilizing the aptamer (Apt) onto the GCE surface. By employing differential pulse voltammetry (DPV) we successfully achieved the detection of chloramphenicol (CAP) with a remarkably low limit of detection of 0.0138 ng mL and the peak DPV currents at 0.18 V (vs. Ag/AgCl) were used for calibration. Furthermore, this aptasensor exhibited high selectivity and reproducibility.
抗生素的过度使用导致了严重的水污染，对人类健康构成了严重威胁。因此，迫切需要识别环境中的抗生素。在这项研究中，我们成功地合成了一种具有大表面积和丰富官能团的空心 PCN-222 MOF，特别是卟啉核。为了提高空心 PCN-222（HPCN-222）的电导率，使用基本的水热法将金（Au）颗粒掺入卟啉核中。这种修饰通过π-π堆积和静电相互作用促进了适体链的有效固定。结果，Au@HPCN-222 复合材料作为将适体（Apt）固定在 GCE 表面的基底表现出优异的效果。通过使用差分脉冲伏安法（DPV），我们成功地实现了对氯霉素（CAP）的检测，检测限低至 0.0138 ng mL，在 0.18 V（相对于 Ag/AgCl）处的峰 DPV 电流用于校准。此外，该适体传感器表现出高选择性和重现性。
