Department of General Medicine, Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia, 010000, China.
Appl Biochem Biotechnol. 2024 Jun;196(6):3076-3087. doi: 10.1007/s12010-023-04714-4. Epub 2023 Aug 24.
The objective of this study is to investigate the expression of the carbohydrate response element binding protein (ChREBP) gene in type 2 diabetes mellitus (T2DM) and its correlation with pathological features. For obtaining and exploring the pathological features in patients, sixty T2DM patients (the research group) and thirty healthy controls (the control group) presented to our hospital between January 2019 and June 2019 were selected as the research participants. After DNA extraction from peripheral blood mononuclear cells (PBMCs) and modification of target gene methylation with bisulfite, differences in methylation were verified, and the correlation of ChREBP methylation level with T2DM pathological features and single nucleotide polymorphism (SNP) typing was discussed. According to the prediction results of UCSC Genome Browser Home, there were two CpG islands in the promoter region of the ChREBP gene, and the first exon was selected for research. The ChREBP methylation rate was statistically higher in the research group versus the control group (P < 0.05). Age, FPG, TC, and TG were confirmed by the multiple linear regression analysis to be correlated with the ChREBP methylation rate (P < 0.05). Finally, there was no difference in ChREBP methylation level between CT- and CC-type patients at rs17145750 and rs1051921 loci (P > 0.05). Peripheral blood ChREBP methylation is elevated in T2DM patients and is closely related to age, blood glucose, and blood-lipid level, which is expected to be a new direction for future T2DM diagnosis and treatment.
本研究旨在探讨碳水化合物反应元件结合蛋白（ChREBP）基因在 2 型糖尿病（T2DM）中的表达及其与病理特征的相关性。为了获取和探索患者的病理特征，我们选择了 2019 年 1 月至 2019 年 6 月期间在我院就诊的 60 例 T2DM 患者（研究组）和 30 名健康对照者（对照组）作为研究对象。从外周血单个核细胞（PBMCs）中提取 DNA，用亚硫酸氢盐对靶基因进行修饰后，验证了甲基化的差异，并讨论了 ChREBP 甲基化水平与 T2DM 病理特征和单核苷酸多态性（SNP）分型的相关性。根据 UCSC Genome Browser Home 的预测结果，ChREBP 基因启动子区有两个 CpG 岛，选择第一外显子进行研究。研究组的 ChREBP 甲基化率明显高于对照组（P<0.05）。多元线性回归分析证实年龄、FPG、TC 和 TG 与 ChREBP 甲基化率相关（P<0.05）。最后，在 rs17145750 和 rs1051921 位点，CT 和 CC 型患者的 ChREBP 甲基化水平无差异（P>0.05）。T2DM 患者外周血 ChREBP 甲基化水平升高，与年龄、血糖和血脂水平密切相关，有望成为未来 T2DM 诊断和治疗的新方向。
