Center for Gene Diagnosis, Zhongnan Hospital, Wuhan University, Hubei, China.
Clin Chim Acta. 2011 Sep 18;412(19-20):1854-60. doi: 10.1016/j.cca.2011.06.024. Epub 2011 Jun 25.
ChREBP regulates lipogenesis and glucose utilization in the liver. Current reports suggest a contradictive association between rs3812316 of this gene and triglyceride level. We hypothesized the polymorphisms in ChREBP gene were associated with CAD in Chinese population.
The ChREBP gene polymorphisms were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods in 200 controls and 310 CAD patients. Serum lipids and glucose concentrations were measured in all subjects. Haplotypes were constructed based on rs3812316, rs7798357 and rs1051921. All the data were analyzed using SPSS14.0, PLINK1.07 and SHEsis software.
The rare allele G of rs3812316 was significantly lower in the CAD group after adjusting for age, sex, BMI, SBP and DBP (OR(a)=0.589, 95%CI=0.361-0.961, P=0.034). No significant differences between cases and controls were found in genotype or allele distributions of rs7798357, rs17145750 and rs1051921. Haplotype CGC was significant higher in CAD group (P<0.01, OR=2.364, 95%CI=1.608-3.474), while haplotypes GGC, CGT, CCC were significant lower in CAD group (P<0.05).
The rs3812316 and the haplotypes in ChREBP gene appeared to be related to high susceptibility to CAD.
ChREBP 调节肝脏的脂肪生成和葡萄糖利用。目前的报告表明，该基因的 rs3812316 与甘油三酯水平之间存在矛盾的关联。我们假设 ChREBP 基因的多态性与中国人群中的 CAD 相关。
采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法分析 200 名对照者和 310 名 CAD 患者的 ChREBP 基因多态性。所有受试者均测量血清脂质和葡萄糖浓度。根据 rs3812316、rs7798357 和 rs1051921 构建单倍型。所有数据均采用 SPSS14.0、PLINK1.07 和 SHEsis 软件进行分析。
校正年龄、性别、BMI、SBP 和 DBP 后，CAD 组 rs3812316 的罕见等位基因 G 明显降低（OR(a)=0.589，95%CI=0.361-0.961，P=0.034）。rs7798357、rs17145750 和 rs1051921 的基因型或等位基因分布在病例组和对照组之间无显著差异。CAD 组 CGC 单倍型明显较高（P<0.01，OR=2.364，95%CI=1.608-3.474），而 CAD 组 GGC、CGT、CCC 单倍型明显较低（P<0.05）。
ChREBP 基因的 rs3812316 和单倍型似乎与 CAD 的高易感性有关。
