Kudo Y, Ozaki K, Miyakawa A, Amano T, Ogura A
Jpn J Pharmacol. 1986 Jul;41(3):345-51. doi: 10.1254/jjp.41.345.
For monitoring the changes in intracellular free Ca2+ concentration ([Ca2+]i), we developed a simple system combining a fluorescence microscope, an image intensifier, a video-camera, a cathode ray tube display and a photodiode, employing quin2 as a Ca2+ indicator. We recorded increases of the fluorescence intensity due to [Ca2+]i rises, when high K+ medium, neurotransmitter and Ca2+ ionophore were applied to the single cells of nervous system origin in culture. The present system is capable of simultaneous detection of the [Ca2+]i changes from multiple separate cells.
为了监测细胞内游离钙离子浓度（[Ca2+]i）的变化，我们开发了一个简单的系统，该系统结合了荧光显微镜、图像增强器、摄像机、阴极射线管显示器和光电二极管，并使用喹啉-2作为钙离子指示剂。当向培养的神经系统来源的单细胞施加高钾培养基、神经递质和钙离子载体时，我们记录了由于[Ca2+]i升高而导致的荧光强度增加。本系统能够同时检测多个独立细胞的[Ca2+]i变化。
