Belen'ky D M, Mikhajlov V I, Rosenfeld E L
Clin Chim Acta. 1979 May 2;93(3):365-70. doi: 10.1016/0009-8981(79)90286-9.
The presence of carbohydrates in homogeneous preparations of human liver acid alpha-glucosidase has been established and the carbohydrate content of the enzyme determined. The enzyme was purified with the specific purpose of removing all low-molecular-weight carbohydrates. It was specifically adsorbed on Concanavalin A-Sepharose, eluted with methyl-alpha-D-mannopyranoside and gave a positive reaction with the phenol-sulphuric acid reagent. These facts taken together provide evidence that the enzyme studied is a glycoprotein. The analysis of the carbohydrate content of human liver acid alpha-glucosidase showed that there were 8.3 glucosamine, 13.2 mannose and possibly 3--4 glucose residues per molecule of the enzyme with a molecular weight of 98,000.
已证实人肝脏酸性α-葡萄糖苷酶的均一制剂中存在碳水化合物，并测定了该酶的碳水化合物含量。该酶经纯化以专门去除所有低分子量碳水化合物。它特异性吸附于伴刀豆球蛋白A-琼脂糖，用甲基-α-D-甘露吡喃糖苷洗脱，并与苯酚-硫酸试剂呈阳性反应。综合这些事实表明所研究的酶是一种糖蛋白。对人肝脏酸性α-葡萄糖苷酶碳水化合物含量的分析表明，每分子分子量为98,000的该酶含有8.3个葡糖胺、13.2个甘露糖，可能还有3 - 4个葡萄糖残基。
