College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Institute of Molecular and Nano Science, Shandong Normal University, Jinan, 250014, PR China.
College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Institute of Molecular and Nano Science, Shandong Normal University, Jinan, 250014, PR China.
Biosens Bioelectron. 2023 Nov 1;239:115584. doi: 10.1016/j.bios.2023.115584. Epub 2023 Aug 9.
MicroRNAs (miRNAs) have emerged as important biomarkers in biomedicine and bioimaging due to their roles in various physiological and pathological processes. Real-time and in situ monitoring of dynamic fluctuation of miRNAs in living cells is crucial for understanding these processes. However, current miRNA imaging probes still have some limitations, including the lack of effective amplification methods for low abundance miRNAs bioanalysis and uncontrollable activation, leading to background signals and potential false-positive results. Therefore, researchers have been integrating activatable devices with miRNA amplification techniques to design stimuli-responsive nanoprobes for "on-demand" and precise imaging of miRNAs in living cells. In this review, we summarize recent advances of stimuli-responsive probes for the amplification-based imaging of miRNAs in living cells and discuss the future challenges and opportunities in this field, aiming to provide valuable insights for accurate disease diagnosis and monitoring.
微小 RNA（miRNA）在各种生理和病理过程中发挥作用，因此成为生物医学和生物成像领域的重要生物标志物。实时和原位监测活细胞中 miRNA 的动态波动对于理解这些过程至关重要。然而，目前的 miRNA 成像探针仍然存在一些局限性，包括缺乏有效的低丰度 miRNA 生物分析放大方法和不可控的激活，导致背景信号和潜在的假阳性结果。因此，研究人员一直在将激活装置与 miRNA 扩增技术相结合，设计对刺激反应的纳米探针，用于“按需”和精确成像活细胞中的 miRNA。在这篇综述中，我们总结了基于刺激的活细胞中 miRNA 成像的刺激响应探针的最新进展，并讨论了该领域的未来挑战和机遇，旨在为准确的疾病诊断和监测提供有价值的见解。
