Department of Emergency medical, The First Affiliated Hospital of Bengbu Medical College, Bengbu, China.
Clin Respir J. 2023 Oct;17(10):1017-1024. doi: 10.1111/crj.13688. Epub 2023 Aug 24.
Acute lung injury (ALI) is a prevalent complication of sepsis with high mortality rate. Saikosaponin D (SSD) is a triterpenoid saponin that has been reported to alleviate sepsis-triggered renal injury in mice. Nonetheless, the therapeutic effect of SSD on sepsis-evoked ALI is unclarified.
Lipopolysaccharide (LPS) from Escherichia coli 055:B5 was utilized to stimulate lung epithelial cell line MLE-12. A mouse model of sepsis was established. CCK-8 assay was employed for determining cytotoxicity. ELISA was utilized for determining proinflammatory cytokine production. Flow cytometry and western blotting were implemented for evaluating cell apoptosis. Hematoxylin-eosin staining was conducted for histologic analysis of murine lung tissues.
SSD alleviated LPS-triggered inflammation and cell apoptosis of MLE-12 cells. SSD treatment ameliorated the pathological damages, inflammatory response, and cell apoptosis in the lungs of septic mice.
SSD protects against sepsis-triggered ALI by inhibiting inflammation and cell apoptosis in MLE-12 cells and septic mouse mice.
急性肺损伤（ALI）是脓毒症的一种常见并发症，死亡率很高。柴胡皂苷 D（SSD）是一种三萜皂苷，据报道可减轻小鼠脓毒症引起的肾损伤。然而，SSD 对脓毒症引起的 ALI 的治疗效果尚不清楚。
采用大肠杆菌 055:B5 的脂多糖（LPS）刺激肺上皮细胞系 MLE-12。建立脓毒症小鼠模型。用 CCK-8 法测定细胞毒性。ELISA 法测定促炎细胞因子的产生。流式细胞术和 Western blot 法评估细胞凋亡。苏木精-伊红（H&E）染色法进行小鼠肺组织的组织学分析。
SSD 减轻了 LPS 诱导的 MLE-12 细胞炎症和细胞凋亡。SSD 治疗改善了脓毒症小鼠肺部的病理损伤、炎症反应和细胞凋亡。
SSD 通过抑制 MLE-12 细胞和脓毒症小鼠的炎症和细胞凋亡来保护 LPS 诱导的 ALI。
