Department of Oncology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, 100 Haining Road, Shanghai, 200080, China.
Department of General Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, 100 Haining Road, Shanghai, 200080, China.
World J Surg Oncol. 2023 Aug 25;21(1):264. doi: 10.1186/s12957-023-03106-6.
To investigate the expression of EBV products and frequency of gallstone disease (GD) among different microsatellite status in colorectal cancer (CRC) with BRAF mutation.
We collected 30 CRC patients with BRAF mutation and 10 BRAF ( -) CRC patients as well as 54 healthy subjects. Tumor tissue samples were collected to detect the mutation of BRAF, KRAS, and TP53. Microsatellite status was determined by immunohistochemistry and PCR. EBER in situ hybridization was performed to detect EBV. In addition, we also collected clinical information about the patients.
We found that although EBV products were detected in CRC, there were no significant differences in the EBV distribution between the different BRAF groups. Our study demonstrated that BRAF mutation and BRAF with MSI were significantly more frequent in the right CRC. Furthermore, the KRAS mutation rate in the BRAF-wild-type group was proved to be significantly higher than that in the BRAF mutation group. In addition, we revealed that BRAF mutation and MSI were independent risk factors of TNM stage. The frequency of GD was higher in CRC patients than in general population, and although there was no significant difference between CRC with or without BRAF mutation, the highest frequency of GD was found in MSS CRC with BRAF mutation.
EBV plays a role in CRC, but is not a determinant of different microsatellite status in CRC with BRAF mutation. The frequency of GD in MSS CRC with BRAF mutation is significantly higher than that in the general population.
研究 BRAF 突变型结直肠癌（CRC）中不同微卫星状态下 EBV 产物的表达和胆石病（GD）的频率。
我们收集了 30 例 BRAF 突变型 CRC 患者和 10 例 BRAF（-）CRC 患者以及 54 例健康对照者。采集肿瘤组织标本，检测 BRAF、KRAS 和 TP53 的突变情况。采用免疫组化和 PCR 法检测微卫星状态。采用 EBER 原位杂交法检测 EBV。此外，还收集了患者的临床资料。
我们发现虽然在 CRC 中检测到 EBV 产物，但不同 BRAF 组之间 EBV 分布无显著差异。我们的研究表明，BRAF 突变和 BRAF 微卫星不稳定性（MSI）在右半 CRC 中更为常见。此外，BRAF 野生型组的 KRAS 突变率明显高于 BRAF 突变组。此外，我们揭示 BRAF 突变和 MSI 是 TNM 分期的独立危险因素。CRC 患者的 GD 发生率高于普通人群，虽然 BRAF 突变型 CRC 与无 BRAF 突变型 CRC 之间无显著差异，但 BRAF 突变型 MSS CRC 的 GD 发生率最高。
EBV 在 CRC 中起作用，但不是 BRAF 突变型 CRC 中不同微卫星状态的决定因素。MSS CRC 伴 BRAF 突变的 GD 发生率明显高于普通人群。
