Anthelmintic activity of aqueous seed extract and its effects on rumen fermentation and methane reduction in Indonesian thin-tailed sheep: An study.

BACKGROUND AND AIM

Seeds from were observed to be abundant as waste. This waste contains active plant compounds whose utilization has not been studied in the livestock sector. This study was conducted to evaluate the potential of an aqueous seed extract of (ASE) as an anthelmintic agent during rumen fermentation and methane reduction.

MATERIALS AND METHODS

Aqueous seed extract of was prepared from cultivated in Wonosobo, Indonesia. Phytochemical analyses were performed to quantify the secondary metabolite content of ASE. adult worm mortality tests, scanning electron microscopy, and gas production tests were conducted to evaluate rumen characteristics, methane reduction, and the potential of ASE as an anthelmintic against . Adult worms and ruminal fluid were collected from Indonesian thin-tailed sheep. Two-way analysis of variance followed by Tukey's test was performed using the Statistical Package for the Social Sciences 21.0 software to detect significant differences.

RESULTS

study results showed that 1-5% ASE inhibited after the 1 h of incubation, and 5% ASE suppressed 100% of adult worms in the 5 h. Scanning electron microscopy analysis of ASE-treated worms ASE revealed damaged cuticle structures. ASE had no significant effect on pH, NH, volatile fatty acid, acetate, propionate, butyrate, acetate: propionate, or microbial protein in rumen fluid (p 0.05). The feed fermentation results showed that ASE significantly affected methane reduction.

CONCLUSION

The inclusion of up to 5% ASE in sheep diets may serve as a potential alternative anthelmintic against as well as a methane reduction agent, without deleterious effects on rumen fermentation.