Key Laboratory of Buffalo Genetics, Breeding and Reproduction Technology, Ministry of Agriculture and Guangxi Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning, 530001, China.
Dairy Science Department, National Research Centre, 33 Bohouth St. Dokki, Giza, 12622, Egypt.
Curr Microbiol. 2020 Jul;77(7):1271-1282. doi: 10.1007/s00284-020-01935-2. Epub 2020 Mar 4.
This study was conducted to evaluate potential of Moringa oleifera seed oil (MOSO) to modulate rumen microflora to mitigate methane (CH) production in different total mixed rations (TMRs). Three TMRs with different roughage (R) to concentrate (C) ratio were used as substrates (R70:C30, R50:C50 and R30:C70) for in vitro fermentation study using batch culture technique. Results revealed that supplementation of MOSO in different rations with variable roughage to concentrates ratio altered (P < 0.05) CH production and fermentation parameters. M. oleifera seed oil at 3 and 4% increased (P < 0.01) microbial protein (MCP) and propionate concentration for all rations but decreased acetate concentration in R70:C30 TMR. Two levels of MOSO (3 and 4%) quadratically increased (P = 0.001) MCP and decreased acetate in R30:C70 while all levels increased propionate. For R30:C70 and R50:C50 rations, MOSO linearly and quadratically decreased (P < 0.001) protozoa and methanogen counts; however, protozoa, methanogens and bacteria were significantly increased in R70:C30 ration without any change in fungal counts. Supplementation of higher level of MOSO (4%) in high roughage ration (R70:C30) showed negative effects on diversity (Shannon index) and evenness of bacterial species as compared to control and lower oil level. Moreover, it also decreased Firmicutes to Bacteroidetes ratio in high roughage rations more obviously at lower levels. Moringa oil also stimulated Prevotella in both high and low roughages diets that indicates its potential to mediate rumen acidosis. Conclusively, MOSO enhanced fermentation kinetics and decreased CH production through effective modulation of rumen microbiome.
本研究旨在评估辣木籽油（MOSO）的潜力，以调节瘤胃微生物群，从而减少不同全混合日粮（TMR）中的甲烷（CH）产量。使用批次培养技术，使用三种不同粗饲料（R）与精料（C）比例的 TMR 作为体外发酵研究的底物：R70:C30、R50:C50 和 R30:C70。结果表明，MOSO 以不同比例的粗饲料和精饲料添加到不同的日粮中，改变了（P < 0.05）CH 产量和发酵参数。M. oleifera 种子油在 3%和 4%时增加（P < 0.01）了所有日粮的微生物蛋白（MCP）和丙酸盐浓度，但降低了 R70:C30 日粮中的乙酸盐浓度。MOSO 的两个水平（3%和 4%）在 R30:C70 中二次增加（P = 0.001）了 MCP，降低了乙酸盐，而所有水平都增加了丙酸盐。对于 R30:C70 和 R50:C50 日粮，MOSO 线性和二次降低（P < 0.001）了原生动物和甲烷菌的数量；然而，在 R70:C30 日粮中，原生动物、甲烷菌和细菌的数量显著增加，真菌数量没有变化。在高粗饲料日粮（R70:C30）中添加较高水平的 MOSO（4%）与对照和较低油水平相比，对细菌种类的多样性（香农指数）和均匀度有负面影响。此外，它还降低了高粗饲料日粮中厚壁菌门与拟杆菌门的比例，而且在较低水平时更为明显。辣木油还刺激了高粗和低粗饲料日粮中的普雷沃氏菌，表明其具有调节瘤胃酸中毒的潜力。总之，MOSO 通过有效调节瘤胃微生物组，增强了发酵动力学，减少了 CH 产量。
