Nano Electronic Center of Excellence, Nano Bio Electronic Devices Laboratory, School of Electrical and Computer Engineering, University of Tehran, Tehran 1439957131, Iran.
Nano Electronic Center of Excellence, Thin Film and Nano Electronics Laboratory, School of Electrical and Computer Engineering, University of Tehran, Tehran 1439957131, Iran.
Biosensors (Basel). 2023 Jul 27;13(8):762. doi: 10.3390/bios13080762.
Here, two different electrode patterns are described as cyclic voltammetry (CV) biosensors to detect the effect of a hypo CO condition (for 6 h) in ambient on cellular secretion. The cells were selected from breast cancer and endothelial standard lines. Changes in CV peaks of the secretions were recorded by the modified pattern whereby increasing the interactive surface with homogenous electric paths was considered by simulation before fabrication. The results of the simulation and experimental procedures showed a meaningful correlation between hypo CO samples and the occurrence of CV oxidative peaks at about 0.07 V and reductive peaks at approximately -0.22 V in the modified biosensor in all cell lines, while no apoptosis was found in any of the control and hypo CO samples. This observation could not be related to the lack of H (alkaline pH induction) in the media solution as such peaks were not observed in the pure cell culture medium but had been maintained in the hypo CO ambient. This approach could be used as a cell-free sensor to monitor ambient shocks. This may not induce apoptosis but may be vital in the proliferation and protein expression of the cells, such as the hypo CO ambient. The sensor is not disposable in use and showed repeatable responses after rinsing.
这里，我们描述了两种不同的电极模式作为循环伏安法（CV）生物传感器，以检测低 CO 环境（6 小时）对细胞分泌的影响。所选细胞来自乳腺癌和内皮标准系。通过改进的模式记录分泌物的 CV 峰变化，该模式在制造前通过模拟考虑了与均匀电场路径的相互作用表面的增加。模拟和实验程序的结果表明，在所有细胞系中，低 CO 样品与在改良生物传感器中约 0.07 V 的 CV 氧化峰和约-0.22 V 的还原峰的发生之间存在有意义的相关性，而在任何对照和低 CO 样品中都未发现细胞凋亡。这种观察结果不能归因于培养基溶液中缺乏 H（碱性 pH 诱导），因为在纯细胞培养基中没有观察到这种峰，但在低 CO 环境中仍保持不变。这种方法可以用作无细胞传感器来监测环境冲击。这可能不会诱导细胞凋亡，但在细胞的增殖和蛋白表达中可能是至关重要的，例如低 CO 环境。该传感器在使用过程中不是一次性的，并且在冲洗后显示可重复的响应。
