Nano Electronic Center of Excellence, Nanobioelectronic Devices Lab., School of Electrical and Computer Eng., Faculty of Engineering, University of Tehran, P.O. Box 14395/515, Tehran, Iran; Nano Electronic Center of Excellence, Nanoelectronics Lab., School of Electrical and Computer Eng.,Faculty of Engineering, University of Tehran, P.O. Box 14395/515, Tehran, Iran.
ATMP Department, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, P.O. BOX 15179/64311, Tehran, Iran.
Biosens Bioelectron. 2019 Oct 1;142:111566. doi: 10.1016/j.bios.2019.111566. Epub 2019 Aug 6.
Cell free diagnosis of cancer is one of the crucial fields in new generation of medical technology. In this regard, cancer detection based on coastal fluids secreted from the tissues (named as secretome) has attracted a lot of attention. Lipids are important macromolecules could be found with much higher concentrations in secretome of cancer tissues vs. normal ones. On the other hand, lipids are the main dielectric components of the secretome with respect to proteins and ions. Here for the first time we introduced an electrochemical lipidomics based on electrical impedance spectroscopy (EIS) of the secretomes to detect the cancerous samples due to the lipidic content of their secretions. The EIS sensor was fabricated by multiwall carbon nanotube (MWCNT) arrays as conductive and super hydrophobic materials to have great interactive surface with the lipidic content of the solution. Results of the tests on the secretions of more than 100 human biopsied breast tissues showed the promising match between the charge transfer resistance (R) of samples' secretions and pathological states of the tissues with meaningful boundary (up to 8 kΩ for normal and more than 13 kΩ for cancer samples). Mass spectroscopic analyses confirmed the higher content of lipids in cancer secretomes. Electrical lipidomics of the secretome shed new lights in cell free cancer diagnosis and could be applied as a complementary clinical approach in all of biopsy based diagnoses in future.
无细胞癌症诊断是新一代医疗技术的关键领域之一。在这方面，基于组织分泌的沿海体液（称为分泌组）的癌症检测引起了广泛关注。脂质是重要的大分子，在癌症组织的分泌组中的浓度比正常组织高得多。另一方面，脂质是分泌组中相对于蛋白质和离子的主要介电成分。在这里，我们首次引入了一种基于电化学脂质组学的基于分泌组的电阻抗谱（EIS）的方法来检测癌症样本，因为它们的分泌物中含有脂质。EIS 传感器由多壁碳纳米管（MWCNT）阵列作为导电和超疏水材料制成，与溶液中的脂质含量具有很大的相互作用表面。对 100 多个人类活检乳腺组织分泌液的测试结果表明，样品分泌液的电荷转移电阻（R）与组织的病理状态之间存在很好的匹配关系，其边界意义重大（正常组织可达 8 kΩ，癌症组织超过 13 kΩ）。质谱分析证实了癌症分泌组中脂质含量较高。分泌组的电脂质组学为无细胞癌症诊断提供了新的思路，并可能在未来的所有基于活检的诊断中作为一种补充的临床方法得到应用。
