Nano Electronic Center of Excellence, Nano Bio Electronic Devices Lab, School of Electrical and Computer Engineering, College of Engineering, University of Tehran, P.O.Box. 14395515, Tehran, Iran.
Department of Physics, Payame Noor University (PNU), P.O.Box 19395-4697, Tehran, Iran.
Sci Rep. 2024 Aug 3;14(1):17986. doi: 10.1038/s41598-024-68297-8.
In order to investigate the changes in the properties of the cell culture solution in the effect of cell synchronization via cell starvation (for 12, 24, and 36 h), a new spiral-interdigital pattern of microelectrode as a biosensor has been proposed. Then, to test its superiority, the results of this spiral-interdigital pattern with the results of the commercial pattern have been compared. The cells were selected from breast cancer standard lines (MDA-MB-231). Changes in CV peaks of the secretions were recorded by the spiral-interdigital pattern, in which increasing the interactive surface with homogenous electric paths had been considered by simulation before fabrication. The results of the simulation and experimental procedures showed a meaningful correlation. The occurrence of CV oxidative peaks at about 0.1-0.4 V and reductive peaks at approximately 0 V in the spiral-interdigital biosensor in the starved MDA-MB-231 cell line has been observed. The starvation situation resembles one that does not cause meaningful cell apoptosis or necrosis, and this method is only used to make the cells synchronized. Also, no peak is observed in normal cell growth conditions. In addition, by using the commercial design of the electrodes, no peak is observed in any of the conditions of normal and synchronized growth of the cells. Therefore, it seems that the observed peaks are caused by the agents that are secreted in the cell culture solution in a synchronized situation. Moreover, the design of the new spiral-interdigital electrode can significantly increase the sensitivity of the sensor to receive these peaks due to more space and a uniform electric field.
为了研究细胞饥饿（12、24 和 36 小时）对细胞同步作用下细胞培养液性质变化的影响，提出了一种新型的螺旋叉指微电极生物传感器。然后，为了测试其优越性，将该螺旋叉指图案的结果与商业图案的结果进行了比较。细胞取自乳腺癌标准系（MDA-MB-231）。通过螺旋叉指图案记录分泌物的 CV 峰变化，在制造前通过模拟考虑了增加与均匀电场相互作用的表面积。模拟和实验过程的结果显示出有意义的相关性。在饥饿的 MDA-MB-231 细胞系中，在螺旋叉指生物传感器中观察到 CV 氧化峰约在 0.1-0.4 V 处和还原峰约在 0 V 处出现。饥饿状态类似于不会导致有意义的细胞凋亡或坏死的状态，这种方法仅用于使细胞同步。此外，在正常细胞生长条件下也观察不到峰。此外，通过使用电极的商业设计，在细胞正常生长和同步生长的任何条件下都观察不到峰。因此，观察到的峰似乎是由同步状态下细胞培养液中分泌的物质引起的。此外，由于具有更多空间和均匀电场，新的螺旋叉指电极设计可以显著提高传感器接收这些峰的灵敏度。
