Interdisciplinary Professional Unit of Engineering, Campus Zacatecas, Instituto Politécnico Nacional, Blvd. del Bote 202 Cerro del Gato Ejido La Escondida, Col. Ciudad Administrativa, Zacatecas 98160, Mexico.
Scientific and Technological Studies Center No. 18, Instituto Politécnico Nacional, Blvd. del Bote 202 Cerro del Gato Ejido La Escondida, Col. Ciudad Administrativa, Zacatecas 98160, Mexico.
Int J Environ Res Public Health. 2023 Aug 18;20(16):6600. doi: 10.3390/ijerph20166600.
Contamination of soils by automotive residual oil represents a global environmental problem. Bioremediation is the technology most suitable to remove this contaminant from the medium. Therefore, this work aimed to evaluate the effectiveness of bioremediation of automotive residual oil-contaminated soils by biostimulation with enzymes, surfactant, and vermicompost. The bioremediation efficiency was examined using a factorial design of 2 to determine the effect of the time, pH and temperature conditions, biostimulation with enzyme-vermicompost, and biostimulation with enzyme-surfactant. Enzymes obtained from L. seeds, commercial vermicompost, and Triton X-100 were used. Results showed that the highest removal efficiency (99.9%) was achieved at 49 days, with a pH of 4.5, temperature of 37 °C, and using biostimulation with enzyme-vermicompost (3% /-5% /). The addition of surfactant was not significant in increasing the removal efficiency. Therefore, the results provide adequate conditions to bioremediate automotive residual oil-contaminated soils by biostimulation using enzymes supported with vermicompost.
汽车剩余油对土壤的污染是一个全球性的环境问题。生物修复是最适合从介质中去除这种污染物的技术。因此，这项工作旨在通过用酶、表面活性剂和蚯蚓堆肥进行生物刺激来评估生物修复受汽车剩余油污染土壤的效果。使用 2 因素析因设计来检查生物修复效率，以确定时间、pH 值和温度条件、酶-蚯蚓堆肥生物刺激和酶-表面活性剂生物刺激的影响。使用了从 L. 种子、商业蚯蚓堆肥和 Triton X-100 中获得的酶。结果表明，在 49 天、pH 值为 4.5、温度为 37°C 且使用酶-蚯蚓堆肥生物刺激（3%/-5%/）的情况下，去除效率最高（99.9%）。表面活性剂的添加并没有显著提高去除效率。因此，结果为通过用酶和蚯蚓堆肥支持的生物刺激来生物修复受汽车剩余油污染的土壤提供了充分的条件。
