Su Qu, Yu Jiang, Fang Kaiqin, Dong Panyue, Li Zheyong, Zhang Wuzhu, Liu Manxia, Xiang Luojing, Cai Junxiong
Hubei Provincial Academy of Eco-EnvironmentSal Science, Wuhan 430070, China.
State Environmental Protection Key Laboratory of Soil Health and Green Remediation, Wuhan 430070, China.
Toxics. 2023 Feb 1;11(2):143. doi: 10.3390/toxics11020143.
The contamination of soils with petroleum and its derivatives is a longstanding, widespread, and worsening environmental issue. However, efforts to remediate petroleum hydrocarbon-polluted soils often neglect or overlook the interference of heavy metals that often co-contaminate these soils and occur in petroleum itself. Here, we identified baumannii strain JYZ-03 according to its Gram staining, oxidase reaction, biochemical tests, and FAME and rDNA gene sequence analyses and determined that it has the ability to degrade petroleum hydrocarbons. It was isolated from soil contaminated by both heavy metals and petroleum hydrocarbons. Strain JYZ-03 utilized diesel oil, long-chain n-alkanes, branched alkanes, and polycyclic aromatic hydrocarbons (PAHs) as its sole carbon sources. It degraded 93.29% of the diesel oil burden in 7 days. It also had a high tolerance to heavy metal stress caused by arsenic (As). Its petroleum hydrocarbon degradation efficiency remained constant over the 0-300 mg/L As(V) range. Its optimal growth conditions were pH 7.0 and 25-30 °C, respectively, and its growth was not inhibited even by 3.0% (/) NaCl. Strain JYZ-03 effectively bioremediates petroleum hydrocarbon-contaminated soil in the presence of As stress. Therefore, strain JYZ-03 may be of high value in petroleum- and heavy-metal-contaminated site bioremediation.
土壤被石油及其衍生物污染是一个长期存在、广泛且日益严重的环境问题。然而，修复石油烃污染土壤的努力往往忽视或忽略了重金属的干扰，这些重金属常常与石油共同污染土壤，并且存在于石油本身之中。在此，我们根据鲍曼不动杆菌菌株JYZ-03的革兰氏染色、氧化酶反应、生化试验以及脂肪酸甲酯（FAME）和核糖体DNA（rDNA）基因序列分析对其进行了鉴定，并确定它具有降解石油烃的能力。它是从同时受到重金属和石油烃污染的土壤中分离出来的。菌株JYZ-03利用柴油、长链正构烷烃、支链烷烃和多环芳烃（PAHs）作为其唯一碳源。它在7天内降解了93.29%的柴油负荷。它对砷（As）引起的重金属胁迫也具有很高的耐受性。在0-300 mg/L的As(V)范围内，其石油烃降解效率保持恒定。其最佳生长条件分别为pH 7.0和25-30°C，即使在3.0%（/）的NaCl存在下其生长也不会受到抑制。菌株JYZ-03在As胁迫存在的情况下能够有效地对石油烃污染土壤进行生物修复。因此，菌株JYZ-03在石油和重金属污染场地的生物修复中可能具有很高的价值。
