Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, People's Republic of China; Key Laboratory of Northwest Water Resource, Environment and Ecology, MOE, Xi'an University of Architecture and Technology, Xi'an, 710055, People's Republic of China.
Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, People's Republic of China; Key Laboratory of Northwest Water Resource, Environment and Ecology, MOE, Xi'an University of Architecture and Technology, Xi'an, 710055, People's Republic of China.
Environ Pollut. 2022 Jan 15;293:118511. doi: 10.1016/j.envpol.2021.118511. Epub 2021 Nov 18.
Petroleum hydrocarbon pollution is a global problem. However, the effects of different petroleum pollution levels on soil microbial communities and ecological functions are still not clear. In this study, we analyzed the changes in microbial community structures and carbon and nitrogen transformation functions in oil-contaminated soils at different concentrations by chemical analysis, high-throughput sequencing techniques, cooccurrence networks, and KEGG database comparison functional gene annotation. The results showed that heavy petroleum concentrations (petroleum concentrations greater than 20,000 mg kg) significantly decreased soil microbial diversity (p = 0.01), soil microbiome network complexity, species coexistence patterns, and prokaryotic carbon and nitrogen fixation genes. In medium petroleum contamination (petroleum concentrations of between 4000 mg kg and 20,000 mg kg), microbial diversity (p > 0.05) and carbon and nitrogen transformation genes showed no evident change but promoted species coexistence patterns. Heavy petroleum contamination increased the Proteobacteria phylum abundance by 3.91%-57.01%, while medium petroleum contamination increased the Actinobacteria phylum abundance by 1.69%-0.26%. The results suggested that petroleum concentrations played a significant role in shifting soil microbial community structures, ecological functions, and species diversities.
石油烃污染是一个全球性问题。然而，不同石油污染水平对土壤微生物群落和生态功能的影响尚不清楚。在这项研究中，我们通过化学分析、高通量测序技术、共现网络和 KEGG 数据库比较功能基因注释，分析了不同浓度石油污染土壤中微生物群落结构和碳氮转化功能的变化。结果表明，高浓度石油（石油浓度大于 20000mgkg）显著降低了土壤微生物多样性（p=0.01）、土壤微生物组网络复杂性、物种共存模式和原核生物的碳氮固定基因。在中浓度石油污染（石油浓度在 4000mgkg 至 20000mgkg 之间）中，微生物多样性（p＞0.05）和碳氮转化基因没有明显变化，但促进了物种共存模式。高浓度石油污染使变形菌门的丰度增加了 3.91%至 57.01%，而中浓度石油污染使放线菌门的丰度增加了 1.69%至 0.26%。研究结果表明，石油浓度对土壤微生物群落结构、生态功能和物种多样性的变化起着重要作用。
