Morales-Mendoza Asunción Guadalupe, Flores-Trujillo Ana Karen Ivanna, Ramírez-Castillo Jesús Adriana, Gallardo-Hernández Salvador, Rodríguez-Vázquez Refugio
Doctoral Program in Nanosciences and Nanotechnology, Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV-IPN), Instituto Politécnico Nacional Avenue, No. 2508, Zacatenco, Mexico City 07360, Mexico.
Department of Biotechnology and Bioengineering, Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV-IPN), Instituto Politécnico Nacional Avenue, No. 2508, Zacatenco, Mexico City 07360, Mexico.
J Fungi (Basel). 2023 Aug 17;9(8):857. doi: 10.3390/jof9080857.
The global environmental issue of arsenic (As) contamination in drinking water is a significant problem that requires attention. Therefore, the aim of this research was to address the application of a sustainable methodology for arsenic removal through mycoremediation aerated with micro-nanobubbles (MNBs), leading to bioscorodite (FeAsO·2HO) generation. To achieve this, the fungus was cultivated in a medium amended with 1 g/L of As(III) and 8.5 g/L of Fe(II) salts at 28 °C for 5 days in a tubular reactor equipped with an air MNBs diffuser (TR-MNBs). A control was performed using shaking flasks (SF) at 120 rpm. A reaction was conducted at 92 °C for 32 h for bioscorodite synthesis, followed by further characterization of crystals through Fourier-Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), and X-ray diffraction (XRD) analyses. At the end of the fungal growth in the TR-MNBs, the pH decreased to 2.7-3.0, and the oxidation-reduction potential (ORP) reached a value of 306 mV at 5 days. Arsenic decreased by 70%, attributed to possible adsorption through rapid complexation of oxidized As(V) with the exchangeable ferrihydrite ((Fe(III))(OH,O)), sites, and the fungal biomass. This mineral might be produced under oxidizing and acidic conditions, with a high iron concentration (As:Fe molar ratio = 0.14). The crystals produced in the reaction using the TR-MNBs culture broth and characterized by SEM, XRD, and FTIR revealed the morphology, pattern, and As-O-Fe vibration bands typical of bioscorodite and römerite (Fe(II)(Fe(III))(SO)·14HO). Arsenic reduction in SF was 30%, with slight characteristics of bioscorodite. Consequently, further research should include integrating the TR-MNBs system into a pilot plant for arsenic removal from contaminated water.
全球饮用水中砷（As）污染这一环境问题十分严峻，亟待关注。因此，本研究旨在探讨一种可持续的方法，即通过微纳米气泡（MNBs）曝气的真菌修复作用去除砷，从而生成臭葱石（FeAsO·2H₂O）。为此，在配备空气微纳米气泡扩散器的管式反应器（TR-MNBs）中，将真菌接种于添加了1 g/L As(III)和8.5 g/L Fe(II)盐的培养基中，于28 °C培养5天。对照组采用摇瓶（SF），转速为120 rpm。在92 °C下反应32 h以合成臭葱石，随后通过傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）和X射线衍射（XRD）分析对晶体进行进一步表征。在TR-MNBs中真菌生长结束时，pH值降至2.7 - 3.0，氧化还原电位（ORP）在5天时达到306 mV。砷含量降低了70%，这可能是由于氧化后的As(V)与可交换的水铁矿（(Fe(III))(OH,O)）位点以及真菌生物质快速络合而实现了吸附。这种矿物可能在氧化和酸性条件下，在高铁浓度（As:Fe摩尔比 = 0.14）时生成。使用TR-MNBs培养液进行反应所生成的晶体，经SEM、XRD和FTIR表征后，呈现出臭葱石和黄钾铁矾（Fe(II)(Fe(III))(SO₄)·14H₂O）典型的形态、图谱及As-O-Fe振动带。摇瓶中砷的去除率为30%，仅有轻微的臭葱石特征。因此，后续研究应包括将TR-MNBs系统集成到中试装置中，用于去除受污染水中的砷。
