Wu Yinggu, Li Xinyu, Dong Li, Liu Tong, Tang Zhengbin, Lin Runmao, Norvienyeku Justice, Xing Mengyu
Key Laboratory of Green Prevention and Control of Tropical Diseases and Pests, Ministry of Education, School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China.
Sanya Nanfan Research Institute, Hainan University, Sanya 572025, China.
J Fungi (Basel). 2023 Aug 21;9(8):863. doi: 10.3390/jof9080863.
The litchi downy blight disease of litchi caused by accounts for severe losses in the field and during storage. While ample quantitative studies have shown that 6-pentyl-2H-pyran-2-one (6PP) possesses antifungal activities against multiple plant pathogenic fungi, the regulatory mechanisms of 6PP-mediated inhibition of fungal pathogenesis and growth are still unknown. Here, we investigated the potential molecular targets of 6PP in the phytopathogenic oomycetes through integrated deployment of RNA-sequencing, functional genetics, and biochemical techniques to investigate the regulatory effects of 6PP against . Previously we demonstrated that 6PP exerted significant oomyticidal activities. Also, comparative transcriptomic evaluation of strains treated with 6PP Revealed significant up-regulations in the expression profile of TOR pathway-related genes, including and the transcription factors . We also noticed that 6PP treatment down-regulated putative negative regulatory genes of the TOR pathway, including and in . Protein-ligand binding analyses revealed stable affinities between PlYY1, PlCytochrome C, PlSpm1, PlrhoH12 proteins, and the 6PP ligand. Phenotypic characterization of targeted gene deletion strains generated in this study using CRISPR/Cas9 and homologous recombination strategies significantly reduced the vegetative growth, sporangium, encystment, zoospore release, and pathogenicity of . These findings suggest that 6PP-mediated activation of expression positively regulates TOR-related responses and significantly influences vegetative growth and the virulence of . The current investigations revealed novel targets for 6PP and underscored the potential of deploying 6PP in developing management strategies for controlling the litchi downy blight pathogen.
由[病原体名称未给出]引起的荔枝霜霉病在田间和储存期间都会造成严重损失。虽然大量定量研究表明，6-戊基-2H-吡喃-2-酮（6PP）对多种植物病原真菌具有抗真菌活性，但6PP介导的抑制真菌发病机制和生长的调控机制仍不清楚。在这里，我们通过综合运用RNA测序、功能遗传学和生化技术，研究了6PP在植物病原卵菌[卵菌名称未给出]中的潜在分子靶点，以探究6PP对[卵菌名称未给出]的调控作用。此前我们证明6PP具有显著的杀卵菌活性。此外，对用6PP处理的[卵菌名称未给出]菌株进行的比较转录组评估显示，TOR途径相关基因的表达谱显著上调，包括[基因名称未给出]和转录因子[转录因子名称未给出]。我们还注意到，6PP处理下调了[卵菌名称未给出]中TOR途径的假定负调控基因，包括[基因名称未给出]和[基因名称未给出]。蛋白质-配体结合分析揭示了PlYY1、Pl细胞色素C、PlSpm1、PlrhoH12蛋白与6PP配体之间的稳定亲和力。本研究使用CRISPR/Cas9和同源重组策略构建的[卵菌名称未给出]靶向基因缺失菌株的表型特征显著降低了[卵菌名称未给出]的营养生长、孢子囊形成、包囊化、游动孢子释放和致病性。这些发现表明，6PP介导的[基因名称未给出]表达激活正向调节TOR相关反应，并显著影响[卵菌名称未给出]的营养生长和毒力。目前的研究揭示了6PP的新靶点，并强调了在制定控制荔枝霜霉病病原体的管理策略中应用6PP的潜力。
