Tao Lingyun, Zhang Yiwen, Li Yaqian, Luo Laipeng, Zhang Zenglu, Chen Jie
School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Shanghai Jiao Tong University, Shanghai 200240, China.
Sheng Wu Gong Cheng Xue Bao. 2020 Jun 25;36(6):1181-1189. doi: 10.13345/j.cjb.190442.
Trichoderma spp. is a kind of filamentous fungi with important biocontrol value. Twelve strains of Trichoderma spp. were isolated from the soils of different types of crops in Shaoxing, Zhejiang and Foshan, Guangdong. The antagonistic resistance to Fusarium oxysporum was compared by plate confrontation test. The further analysis of volatile secondary metabolites for two strains were carried out using HS-SPME-GC-MS analysis. The results showed that T. asperellum ZJSX5003 and GDFS1009 had fast growth ability, and the inhibition effects on F. oxysporum were 73% and 74% respectively. Six identical volatile metabolites were detected as follows 2-Methyl-1-propanol, 3-Methyl-1-butanol, 3-Methyl-3-buten-1-ol, Acetyl methyl carbinol, Butane-2,3-diol and 6-n-pentyl-2H-pyran-2-one (6-PAP). Among them, 6-PAP was validated to have a higher inhibitory effect on F. oxysporum in vitro. This study will provide basis for the development of biocontrol agents with metabolites of Trichoderma, such as 6-PAP.
木霉菌是一类具有重要生防价值的丝状真菌。从浙江绍兴和广东佛山不同类型农作物土壤中分离出12株木霉菌株。通过平板对峙试验比较了它们对尖孢镰刀菌的拮抗抗性。利用顶空固相微萃取-气相色谱-质谱联用（HS-SPME-GC-MS）分析方法对其中两株菌株的挥发性次生代谢产物进行了进一步分析。结果表明，棘孢木霉ZJSX5003和绿色木霉GDFS1009生长能力较快，对尖孢镰刀菌的抑制率分别为73%和74%。检测到6种相同的挥发性代谢产物，分别为2-甲基-1-丙醇、3-甲基-1-丁醇、3-甲基-3-丁烯-1-醇、乙酰甲基甲醇、丁烷-2,3-二醇和6-正戊基-2H-吡喃-2-酮（6-PAP）。其中，经证实6-PAP在体外对尖孢镰刀菌具有较高的抑制作用。本研究将为开发以木霉菌代谢产物如6-PAP为基础的生防制剂提供依据。
