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基于Neuro-2a 细胞的测定法评估河豚毒素类似物的毒性等效因子及其在希腊河豚鱼中的应用。

Evaluation of Toxicity Equivalency Factors of Tetrodotoxin Analogues with a Neuro-2a Cell-Based Assay and Application to Puffer Fish from Greece.

机构信息

Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Ctra. Poble Nou km 5.5, 43540 La Ràpita, Spain.

Institute of Marine Biology, Biotechnology and Aquaculture, Hellenic Centre for Marine Research, 71003 Heraklion, Greece.

出版信息

Mar Drugs. 2023 Jul 29;21(8):432. doi: 10.3390/md21080432.

DOI:10.3390/md21080432
PMID:37623713
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10455759/
Abstract

Tetrodotoxin (TTX) is a potent marine neurotoxin involved in poisoning cases, especially through the consumption of puffer fish. Knowledge of the toxicity equivalency factors (TEFs) of TTX analogues is crucial in monitoring programs to estimate the toxicity of samples analyzed with instrumental analysis methods. In this work, TTX analogues were isolated from the liver of a individual caught on South Crete coasts. A cell-based assay (CBA) for TTXs was optimized and applied to the establishment of the TEFs of 5,11-dideoxyTTX, 11-norTTX-6()-ol, 11-deoxyTTX and 5,6,11-trideoxyTTX. Results showed that all TTX analogues were less toxic than the parent TTX, their TEFs being in the range of 0.75-0.011. Then, different tissues of three individuals were analyzed with CBA and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The obtained TEFs were applied to the TTX analogues' concentrations obtained by LC-MS/MS analysis, providing an indication of the overall toxicity of the sample. Information about the TEFs of TTX analogues is valuable for food safety control, allowing the estimation of the risk of fish products to consumers.

摘要

河豚毒素（TTX）是一种强效海洋神经毒素，与中毒事件有关，特别是通过食用河豚鱼导致中毒。了解 TTX 类似物的毒性当量因子（TEFs）对于监测计划至关重要，可用于评估使用仪器分析方法分析的样本的毒性。在这项工作中，从在克里特岛南部海岸捕获的个体的肝脏中分离出 TTX 类似物。优化了用于 TTX 的基于细胞的测定法（CBA），并将其应用于建立 5,11-去氧TTX、11-去甲 TTX-6()-醇、11-去氧 TTX 和 5,6,11-三去氧 TTX 的 TEFs。结果表明，所有 TTX 类似物均比母体 TTX 的毒性低，其 TEFs 范围为 0.75-0.011。然后，使用 CBA 和液相色谱-串联质谱法（LC-MS/MS）分析了三个个体的不同组织。应用 CBA 获得的 TEFs 对 LC-MS/MS 分析得到的 TTX 类似物浓度进行了分析，为样品的整体毒性提供了指示。了解 TTX 类似物的 TEFs 对于食品安全控制非常有价值，可以估计鱼产品对消费者的风险。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aae/10455759/ada0491fecd2/marinedrugs-21-00432-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aae/10455759/cc9ad07aadbf/marinedrugs-21-00432-g0A1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aae/10455759/933e4cea26f5/marinedrugs-21-00432-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aae/10455759/bdcc2786ab03/marinedrugs-21-00432-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aae/10455759/1b2966480e4e/marinedrugs-21-00432-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aae/10455759/4739d2b361c1/marinedrugs-21-00432-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aae/10455759/ea02ee102172/marinedrugs-21-00432-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aae/10455759/ada0491fecd2/marinedrugs-21-00432-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aae/10455759/cc9ad07aadbf/marinedrugs-21-00432-g0A1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aae/10455759/933e4cea26f5/marinedrugs-21-00432-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aae/10455759/bdcc2786ab03/marinedrugs-21-00432-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aae/10455759/1b2966480e4e/marinedrugs-21-00432-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aae/10455759/4739d2b361c1/marinedrugs-21-00432-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aae/10455759/ea02ee102172/marinedrugs-21-00432-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aae/10455759/ada0491fecd2/marinedrugs-21-00432-g006.jpg

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