IRTA, Ctra. Poble Nou, km 5.5, 43540, Sant Carles de la Ràpita, Tarragona, Spain.
IRTA, Ctra. Poble Nou, km 5.5, 43540, Sant Carles de la Ràpita, Tarragona, Spain.
Food Chem Toxicol. 2020 Jun;140:111315. doi: 10.1016/j.fct.2020.111315. Epub 2020 Apr 5.
Tetrodotoxin (TTX) is a potent neurotoxin responsible for many food poisoning incidents and some fatalities. Although mainly associated with the consumption of pufferfish, in recent years, TTX has been found in shellfish, particularly in Europe. In this work, a magnetic bead (MB)-based colorimetric immunoassay was applied to the detection of TTX in Pacific oysters (Crassostrea gigas), razor clams (Solen marginatus) and mussels (Mytilus galloprovincialis). Effective LODs (eLODs) for TTX of 1 μg/kg in oysters and razor clams and 3.3 μg/kg in mussels, significantly below the EFSA guidance threshold (44 μg/kg), were obtained. The strategy was applied to the analysis of naturally-contaminated Pacific oysters (Crassostrea gigas) and mussels (Mytilus edulis) from the Netherlands, and TTX was detected in all samples. The approach, which takes less than 1.5 h, proved to be useful as a rapid and simple method to detect TTX, support shellfish safety and protect consumers.
河豚毒素（TTX）是一种强效神经毒素，可导致许多食物中毒事件和一些死亡事件。尽管主要与食用河豚鱼有关，但近年来，TTX 已在贝类中发现，尤其是在欧洲。在这项工作中，应用基于磁珠（MB）的比色免疫测定法检测太平洋牡蛎（Crassostrea gigas）、刀贝（Solen marginatus）和贻贝（Mytilus galloprovincialis）中的 TTX。在牡蛎和刀贝中，TTX 的有效检测限（eLOD）为 1 μg/kg，在贻贝中为 3.3 μg/kg，明显低于 EFSA 指导阈值（44 μg/kg）。该策略应用于分析来自荷兰的天然污染太平洋牡蛎（Crassostrea gigas）和贻贝（Mytilus edulis），并在所有样品中均检测到 TTX。该方法耗时不到 1.5 小时，被证明是一种快速简便的 TTX 检测方法，可支持贝类安全并保护消费者。
