Grippi Francesca, Blanda Valeria, Galluzzo Paola, Bongiorno Manuel, Sciacca Carmela, Arcuri Francesca, D'Agostino Rosalia, Giacchino Ilenia, Gucciardi Francesca, D'Incau Mario, Bertasio Cristina, Torina Alessandra, Guercio Annalisa
Istituto Zooprofilattico Sperimentale della Sicilia, Via Gino Marinuzzi 3, 90129 Palermo, Italy.
Dipartimento di Prevenzione Veterinaria-Area di Sanità Pubblica Veterinaria, Unità Operativa Territoriale Alcamo Castelvetrano, Via Sardegna, 91022 Castelvetrano, Italy.
Vet Sci. 2023 Aug 6;10(8):508. doi: 10.3390/vetsci10080508.
Leptospirosis is a worldwide widespread zoonosis caused by genus. We report an acute leptospirosis case in a puppy housed at a municipal kennel and the subsequent diagnostic investigations carried out on all dogs housed in the kennel. Laboratory investigation included mainly a microagglutination test, real-time PCR, and multi-locus sequence typing (MLST) for genus. Other agents of infection were excluded. The puppy resulted positive for Icterohaemorrhagiae both with serological and molecular assays. All of the other 66 dogs in the kennel underwent clinical and laboratory investigations twice, 15 days apart. No other dog showed leptospirosis clinical signs. At the first sampling, eight dogs (12%) showed antibodies against serogroup Icterohaemorragiae serovar Copenhageni. Real-time PCR on urine samples of seropositive dogs detected spp. DNA in one sample, then identified as serogroup Icterohaemorragiae by MLST. Fifteen days after, four of the previous seropositive dogs still showed antibodies against spp. All urine samples collected from seropositive dogs were negative at real-time PCR. The study allowed the early confirmation of a Leptospirosis case and the identification of at least one asymptomatic carrier of pathogenic spp. The prompt activation of all appropriate management measures allowed limiting and extinguishing the infection.
钩端螺旋体病是一种由钩端螺旋体属引起的全球广泛传播的人畜共患病。我们报告了一例发生在市立犬舍一只幼犬身上的急性钩端螺旋体病病例，以及随后对犬舍内所有犬只进行的诊断调查。实验室调查主要包括针对钩端螺旋体属的显微凝集试验、实时荧光定量聚合酶链反应（PCR）和多位点序列分型（MLST）。排除了其他感染病原体。该幼犬通过血清学和分子检测均显示出血清型黄疸出血群阳性。犬舍内其他66只犬均接受了两次临床和实验室检查，间隔15天。没有其他犬表现出钩端螺旋体病的临床症状。在第一次采样时，8只犬（12%）显示出针对黄疸出血血清群哥本哈根血清型的抗体。对血清学阳性犬尿液样本进行的实时荧光定量PCR在一份样本中检测到钩端螺旋体属DNA，随后通过MLST鉴定为黄疸出血血清群。15天后，之前血清学阳性的4只犬仍显示出针对钩端螺旋体属的抗体。从血清学阳性犬采集的所有尿液样本在实时荧光定量PCR检测中均为阴性。该研究实现了对一例钩端螺旋体病病例的早期确诊，并鉴定出至少一例致病性钩端螺旋体属的无症状携带者。迅速启动所有适当的管理措施得以控制和消除感染。
