浓缩生长因子联合脂肪移植在修复颜面部软组织凹陷畸形中的应用研究。
Study on the Application of Concentrated Growth Factor Combined With Adipose Transplantation in Repairing Depressed Deformities of Soft Tissue in the Face.
机构信息
From the Department of Plastic and Reconstructive Surgery, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui Province, PR China.
出版信息
Ann Plast Surg. 2023 Oct 1;91(4):485-492. doi: 10.1097/SAP.0000000000003666. Epub 2023 Aug 19.
BACKGROUND
Autologous fat is a rich source of adipose tissue that is safe for transplantation. Decreasing the long-term absorption rate is key to improve the survival of transplanted adipose tissue. The aim of this study was to assess the effect of concentrated growth factor (CGF) on the survival of transplanted adipose tissue for repair of facial depression malformations.
METHODS
Coleman adipose granules (CAGs) were prepared from venous blood. In the animal experiment, the ears of 30 healthy male New Zealand white rabbits were randomly allocated to 1 of 4 groups: CGF + CAG (CGF group), platelet-rich fibrin (PRF) + CAG (PRF group), CAG alone (CAG group), and adipose granule transplantation group (control group). Postoperative survival of the transplanted adipose tissue was assessed, the survival and absorption rates of adipose were calculated, and immunohistochemical analysis of specimens was conducted by staining with hematoxylin and eosin and Oil Red O. Of 43 outpatients, 22 received simple adipose transplantation and 21 received autologous CGF combined with adipose transplantation. The adipose absorption rate, complication rate, and cosmetic improvement of the 2 groups were compared.
RESULTS
More adipocytes that are normal were observed in the CGF group, with fewer vacuoles and more uniform distribution of adipose tissue. Survival of the adipose tissue was superior in the CGF and PRF groups. Meanwhile, vascular density and long-term stability were better in the CGF group than the PRF group. In terms of clinical efficacy, the uniformity and survival rate of the adipose tissue were relatively improved in the CGF group compared with the simple adipose particle transplantation group, with less early liquefaction.
CONCLUSION
Concentrated growth factor stabilized and improved the survival of transplanted adipose tissue for filling of facial depression malformations.
背景
自体脂肪是一种安全的移植用脂肪组织来源，降低长期吸收率是提高移植脂肪组织存活率的关键。本研究旨在评估浓缩生长因子（CGF）对修复面部凹陷畸形的移植脂肪组织存活的影响。
方法
从静脉血中制备 Coleman 脂肪颗粒（CAG）。在动物实验中，将 30 只健康雄性新西兰白兔的耳朵随机分配到以下 4 组之一：CGF+CAG（CGF 组）、富血小板纤维蛋白（PRF）+CAG（PRF 组）、CAG 单独组（CAG 组）和脂肪颗粒移植组（对照组）。评估移植脂肪组织的存活情况，计算脂肪的存活率和吸收率，并通过苏木精和伊红及油红 O 染色对标本进行免疫组织化学分析。43 例门诊患者中，22 例行单纯脂肪移植，21 例行自体 CGF 联合脂肪移植。比较两组的脂肪吸收率、并发症发生率和美容改善情况。
结果
CGF 组观察到更多正常的脂肪细胞，空泡较少，脂肪组织分布更均匀。CGF 和 PRF 组的脂肪组织存活率更高。同时，CGF 组的血管密度和长期稳定性优于 PRF 组。在临床疗效方面，CGF 组与单纯脂肪颗粒移植组相比，脂肪组织的均匀性和存活率相对提高，早期液化较少。
结论
浓缩生长因子稳定并提高了移植脂肪组织用于填充面部凹陷畸形的存活率。
Zotero 插件