Hu Yun, Jiang Yichen, Wang Muyao, Tian Weidong, Wang Hang
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, P.R.China.
Department of Oral and Maxillofacial Surgery, West China School of Stomatology, Sichuan University, Chengdu, P.R.China.
Dermatol Surg. 2018 Jul;44(7):976-984. doi: 10.1097/DSS.0000000000001337.
Concentrated growth factors (CGFs) belong to a new generation biomaterials that concentrate large number of growth factors and CD34 stem cells in small volume of plasma.
The purpose of this study was to evaluate the impact of the new technique, CGF, on fat graft survival, which compared with platelet-rich plasma (PRP) and platelet-rich fibrin (PRF).
Nude mice received fat graft were divided into PRP group, PRF group, CGF group, and saline. The grafts were volumetrically and histologically evaluated at 4, 8, and 12 weeks after fat grafting. In vitro growth factor levels in PRP, PRF, and CGF were compared using enzyme-linked immunoassay method. Cell count and real-time polymerase chain reaction were used to evaluate the impact of CGF in medium on human adipose-derived stem cell (hADSC) proliferation and vascular differentiation, respectively.
Fat graft weight was significantly higher in the CGF group than those in the other groups, and histologic evaluation revealed greater vascularity, fewer cysts, and less fibrosis. Adding CGF to the medium maximally promoted hADSC proliferation and expressing vascular endothelial growth factor and PECAM-1.
In this preliminary study, CGF treatment improved the survival and quality of fat grafts.
浓缩生长因子（CGFs）属于新一代生物材料，能在少量血浆中浓缩大量生长因子和CD34干细胞。
本研究旨在评估新技术CGF对脂肪移植存活的影响，并与富血小板血浆（PRP）和富血小板纤维蛋白（PRF）进行比较。
接受脂肪移植的裸鼠分为PRP组、PRF组、CGF组和生理盐水组。在脂肪移植后4周、8周和12周对移植物进行体积和组织学评估。采用酶联免疫吸附测定法比较PRP、PRF和CGF中体外生长因子水平。分别采用细胞计数和实时聚合酶链反应评估CGF在培养基中对人脂肪干细胞（hADSC）增殖和血管分化的影响。
CGF组脂肪移植重量显著高于其他组，组织学评估显示血管更丰富、囊肿更少、纤维化更少。向培养基中添加CGF最大程度地促进了hADSC增殖，并表达血管内皮生长因子和血小板内皮细胞黏附分子-1。
在这项初步研究中，CGF治疗改善了脂肪移植的存活率和质量。
