Giannotti Laura, Calabriso Nadia, Spedicato Francesco, Palermo Andrea, Di Chiara Stanca Benedetta, Demitri Christian, De Sangro Maria Antonietta, Carluccio Maria Annunziata, Damiano Fabrizio, Siculella Luisa, Stanca Eleonora
Department of Experimental Medicine (DiMeS), University of Salento, 73100 Lecce, Italy.
Institute of Clinical Physiology (IFC), National Research Council (CNR), 73100 Lecce, Italy.
Int J Mol Sci. 2025 Sep 5;26(17):8646. doi: 10.3390/ijms26178646.
Concentrated growth factor (CGF) is an autologous blood-derived product widely used in regenerative medicine due to its high concentration of growth factors and platelets. In this study, the ability of primary stem cells isolated from human CGF to differentiate into adipocytes, endothelial cells, and neuronal-like cells was evaluated in vitro. CGF primary cells (CPCs) were obtained from CGF fragments and characterized after one month in culture. These cells were positive for the surface markers CD105, CD45, CD31, and CD14, and also expressed mRNA levels of the stemness markers Nanog and Oct3/4 comparable to human bone marrow mesenchymal stem cells (BMSCs). Results showed that, following appropriate differentiation protocols, CPCs, similarly to BMSCs, were able to differentiate into adipogenic, endothelial, and neuronal lineages, acquiring specific phenotypic and molecular markers. Adipogenic induction resulted in lipid accumulation and the upregulation of key genes, including PLIN2, FABP4, CD36, and FASN. Under pro-endothelial conditions, the cells exhibited increased expression of endothelial markers, eNOS, VEGFR-2, and CD31. Neuronal induction promoted the expression of β-tubulin III, Nestin, and Neurofilament. Overall, this work highlights the remarkable plasticity of CPCs and supports their potential application in multilineage regenerative therapies.
浓缩生长因子（CGF）是一种自体血液衍生产品，因其高浓度的生长因子和血小板而广泛应用于再生医学。在本研究中，评估了从人CGF中分离的原代干细胞在体外分化为脂肪细胞、内皮细胞和神经元样细胞的能力。CGF原代细胞（CPCs）从CGF片段中获得，并在培养一个月后进行表征。这些细胞的表面标志物CD105、CD45、CD31和CD14呈阳性，并且其干性标志物Nanog和Oct3/4的mRNA水平与人类骨髓间充质干细胞（BMSCs）相当。结果表明，按照适当的分化方案，CPCs与BMSCs类似，能够分化为脂肪生成、内皮和神经元谱系，获得特定的表型和分子标志物。脂肪生成诱导导致脂质积累以及关键基因（包括PLIN2、FABP4、CD36和FASN）的上调。在促内皮条件下，细胞表现出内皮标志物eNOS、VEGFR-2和CD31的表达增加。神经元诱导促进了β-微管蛋白III、巢蛋白和神经丝的表达。总体而言，这项工作突出了CPCs显著的可塑性，并支持它们在多谱系再生治疗中的潜在应用。
