School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, PR China; Institute for Inheritance-Based Innovation of Chinese Medicine, Marshall Laboratory of Biomedical Engineering, School of Pharmacy, Shenzhen University Medical School, Shenzhen University, Shenzhen 518055, PR China.
Institute for Inheritance-Based Innovation of Chinese Medicine, Marshall Laboratory of Biomedical Engineering, School of Pharmacy, Shenzhen University Medical School, Shenzhen University, Shenzhen 518055, PR China.
Phytochemistry. 2023 Nov;215:113835. doi: 10.1016/j.phytochem.2023.113835. Epub 2023 Aug 23.
An unprecedented dimeric abietane, succipenoid A (1), and two previously undescribed nor-abietane diterpenoids featuring a rarely occurring naphthalene ring or with a large conjugated system, succipenoids B and C (2 and 3), along with seven known diterpenoids (4-10) were isolated from the CHCl extract of succinum. The structures of these compounds, including their absolute configurations, were elucidated using spectroscopic and computational techniques. Notably, compounds 1-4 and 6-10 were isolated from succinum for the first time. In order to evaluate their anti-inflammatory potential, in vitro tests were conducted. The results demonstrated that compounds 1, 2, 4, and 6-10 exhibite dose-dependent inhibition of iNOS expression in lipopolysaccharide-induced RAW 264.7 cells.
从 succinum 的 CHCl3 提取物中分离得到了一种前所未有的二聚 abietane、 succipenoid A（1），以及两种以前未描述过的 nor-abietane 二萜，它们具有罕见的萘环或大共轭体系，succipenoids B 和 C（2 和 3），以及七种已知的二萜（4-10）。这些化合物的结构，包括它们的绝对构型，是使用光谱和计算技术阐明的。值得注意的是，化合物 1-4 和 6-10 是首次从 succinum 中分离得到的。为了评估它们的抗炎潜力，进行了体外测试。结果表明，化合物 1、2、4 和 6-10 在脂多糖诱导的 RAW 264.7 细胞中表现出剂量依赖性的 iNOS 表达抑制作用。
