D'Alvia Livio, Peruzzi Barbara, Apa Ludovica, Del Prete Zaccaria, Rizzuto Emanuele
Department of Mechanical and Aerospace Engineering, Sapienza University of Rome, 00184 Rome, Italy.
Bone Physiopathology Research Unit, Bambino Gesù Children's Hospital, IRCCS, 00146 Rome, Italy.
Bioengineering (Basel). 2023 Jul 31;10(8):907. doi: 10.3390/bioengineering10080907.
This paper presents a measurement procedure for analyzing the dielectric properties of cells using descriptive statistics. The study focuses on four cancer cell lines (MDA-MB-231 and MCF-7 breast cancer, SaOS-2, and 143B osteosarcoma) and DMEM culture medium, utilizing the Lorentzian fit model of the return-loss function. The measurements are performed using a circular patch resonator with a 40 mm diameter, powered by a miniVNA operating in the frequency range of 1 MHz to 3 GHz. Eight specimens are prepared for each group to ensure reliability, and the return loss is recorded ten times for each specimen. Various statistical parameters are calculated and evaluated, including the average value, standard deviation, coefficient of variation, and relative error between the average and the first values. The results demonstrate that one single acquisition highly represents the entire set of ten data points, especially for the resonant frequency, with an accuracy error lower than 0.05%. These findings have significant implications for the methodological approach to detecting cells' dielectric properties, as they substantially reduce time and preserve the specimens without compromising the accuracy of the experimental results.
本文介绍了一种使用描述性统计分析细胞介电特性的测量程序。该研究聚焦于四种癌细胞系（MDA-MB-231和MCF-7乳腺癌细胞系、SaOS-2细胞系以及143B骨肉瘤细胞系）和DMEM培养基，采用回波损耗函数的洛伦兹拟合模型。测量使用直径为40毫米的圆形贴片谐振器进行，由工作在1 MHz至3 GHz频率范围内的miniVNA供电。每组准备八个样本以确保可靠性，每个样本的回波损耗记录十次。计算并评估了各种统计参数，包括平均值、标准差、变异系数以及平均值与首个值之间的相对误差。结果表明，单次采集高度代表了十个数据点的整个集合，特别是对于谐振频率，精度误差低于0.05%。这些发现对检测细胞介电特性的方法学具有重要意义，因为它们大幅减少了时间并保存了样本，同时不影响实验结果的准确性。
