College of Animal Science, Anhui Science and Technology University, No. 9 Donghua Road, Fengyang County, Chuzhou 233100, China.
Anhui Province Key Laboratory of Animal Nutritional Regulation and Health, No. 9 Donghua Road, Fengyang County, Chuzhou 233100, China.
Genes (Basel). 2023 Jul 30;14(8):1560. doi: 10.3390/genes14081560.
The proper supplementation of boron, an essential trace element, can enhance animal immune function. We utilized the method of TMT peptide labeling in conjunction with LC-MS/MS quantitative proteomics for the purpose of examining the effects of boric acid on a rat model and analyzing proteins from the duodenum. In total, 5594 proteins were obtained from the 0, 10, and 320 mg/L boron treatment groups. Two hundred eighty-four proteins that exhibit differential expression were detected. Among the comparison, groups of 0 vs. 10 mg/L, 0 vs. 320 mg/L, and 10 vs. 320 mg/L of boron, 110, 32, and 179 proteins, respectively, demonstrated differential expression. The results revealed that these differential expression proteins (DEPs) mainly clustered into two profiles. GO annotations suggested that most of the DEPs played a role in the immune system process, in which 2'-5'-oligoadenylate synthetase-like, myxovirus resistance 1, myxovirus resistance 2, dynein cytoplasmic 1 intermediate chain 1, and coiled-coil domain containing 88B showed differential expression. The DEPs had demonstrated an augmentation in the signaling pathways, which primarily include phagosome, antigen processing, and presentation, as well as cell adhesion molecules (CAMs). Our study found that immune responses in the duodenum were enhanced by lower doses of boron and that this effect is likely mediated by changes in protein expression patterns in related signaling pathways. It offers an in-depth understanding of the underlying molecular mechanisms that lead to immune modulation in rats subjected to dietary boron treatment.
硼作为一种必需的微量元素，适当补充可以增强动物的免疫功能。我们采用 TMT 肽标记联合 LC-MS/MS 定量蛋白质组学的方法，研究了硼酸对大鼠模型的影响，并分析了十二指肠的蛋白质。从 0、10 和 320mg/L 硼处理组中获得了 5594 种蛋白质。检测到 284 种差异表达的蛋白质。在比较中，0 与 10mg/L、0 与 320mg/L 和 10 与 320mg/L 硼组分别有 110、32 和 179 种蛋白质差异表达。结果表明，这些差异表达蛋白（DEPs）主要分为两种类型。GO 注释表明，大多数 DEPs 参与了免疫系统过程，其中 2'-5'-寡腺苷酸合成酶样、流感病毒抗性 1、流感病毒抗性 2、动力蛋白细胞质 1 中间链 1 和卷曲螺旋结构域包含 88B 差异表达。DEPs 增强了信号通路，主要包括吞噬体、抗原加工和呈递以及细胞黏附分子（CAMs）。我们的研究发现，较低剂量的硼可以增强十二指肠的免疫反应，这种作用可能是通过相关信号通路中蛋白质表达模式的变化介导的。它深入了解了膳食硼处理大鼠免疫调节的潜在分子机制。
