Department of Molecular Biology, National Institute of Geriatrics, Rheumatology and Rehabilitation, 02-637 Warsaw, Poland.
Department of Geriatrics, National Institute of Geriatrics, Rheumatology and Rehabilitation, 02-637 Warsaw, Poland.
Int J Mol Sci. 2023 Aug 17;24(16):12885. doi: 10.3390/ijms241612885.
Prostaglandin signaling pathways are closely related to inflammation, but also muscle regeneration and processes associated with frailty and sarcopenia, whereas β-catenin ( gene) as a part of Wnt signaling is also involved in the differentiation of muscle cells and fibrosis. The present study analyzed the association between selected prostaglandin pathway genes and clinical parameters in patients with sarcopenia and frailty syndrome. The present study was conducted on patients with sarcopenia, frailty syndrome, and control older patients (N = 25). Additionally, two healthy controls at the age of 25-30 years (N = 51) and above 50 years old (N = 42) were included. The expression of the , (), , and genes in whole blood was checked by the qPCR method. The serum cytokine levels (IL-10, TNFα, IFN-y, IL-1α, IL-1β) in patients and controls were checked by the Q-Plex Human Cytokine Panel. The results showed a significant effect of age on gene expression ( = 0.01). A negative trend between the appendicular skeletal muscle mass parameter (ASSM) and the expression of has been noted (r = -0.224, = 0.484). and expressions negatively correlated with creatine phosphokinase (r = -0.71, = 0.009; r = -0.58, = 0.047) and positively with the functional mobility test timed up and go scale (TUG) (r = 0.61, = 0.04; r = 0.63, = 0.032). In the older control group, a negative association between iron levels and the expression of -0.47, = 0.017) was observed. A similar tendency was noted in patients with sarcopenia (r = -0.112, = 0.729). A negative trend between appendicular skeletal muscle mass (ASMM) and seems to confirm the impairment of muscle regeneration associated with sarcopenia. The expression of the studied genes revealed a trend in associations with the clinical picture of muscular dystrophy and weakening patients. Perhaps and is in opposition to the role of the receptor in muscle physiology. Nevertheless, further, including functional studies is needed.
前列腺素信号通路与炎症密切相关，但也与肌肉再生以及与虚弱和肌肉减少症相关的过程有关，而 β-连环蛋白（基因）作为 Wnt 信号的一部分，也参与肌肉细胞的分化和纤维化。本研究分析了选定的前列腺素通路基因与肌肉减少症和虚弱综合征患者的临床参数之间的关系。本研究在肌肉减少症、虚弱综合征和对照老年患者（N=25）中进行。此外，还纳入了年龄在 25-30 岁（N=51）和 50 岁以上（N=42）的两名健康对照者。通过 qPCR 法检查全血中 、 、 、 基因的表达。通过 Q-Plex 人类细胞因子面板检查患者和对照者的血清细胞因子水平（IL-10、TNFα、IFN-y、IL-1α、IL-1β）。结果显示，年龄对 基因表达有显著影响（ = 0.01）。已经注意到四肢骨骼肌质量参数（ASSM）与 表达之间存在负趋势（r = -0.224， = 0.484）。 和 表达与肌酸磷酸激酶呈负相关（r = -0.71， = 0.009；r = -0.58， = 0.047），与功能性移动测试计时起跑（TUG）呈正相关（r = 0.61， = 0.04；r = 0.63， = 0.032）。在老年对照组中，观察到铁水平与 表达之间存在负相关（r = -0.47， = 0.017）。在肌肉减少症患者中也观察到类似的趋势（r = -0.112， = 0.729）。四肢骨骼肌质量（ASMM）与 之间的负趋势似乎证实了与肌肉减少症相关的肌肉再生受损。研究基因的表达显示出与肌肉疾病和虚弱患者临床症状相关的趋势。也许 和 与肌肉生理学中的 受体作用相反。然而，需要进一步包括功能研究。
