Doherty P, Dickson J G, Flanigan T P, Kennedy P G, Walsh F S
Neurology. 1986 Oct;36(10):1330-4. doi: 10.1212/wnl.36.10.1330.
We used a quantitative immunoassay to examine the effects of human serum and immunoglobulins on neurofilament protein expression in cultures of chick spinal neurons. Compared with cultures grown in the presence of serum from healthy controls or patients with other neurologic disorders, ALS serum lowered the level of neurofilament proteins. Effects were similar with or without muscle-derived neurotrophic factors; there was no specificity for motor neurons. No neurotoxic activity was found in immunoglobulin fractions, and there was no evidence of circulating antibodies that might neutralize muscle-derived neurotrophic factors or induce cytolysis of spinal neurons.
我们采用定量免疫测定法，研究人血清和免疫球蛋白对鸡脊髓神经元培养物中神经丝蛋白表达的影响。与在来自健康对照者或患有其他神经系统疾病患者的血清存在下培养的细胞相比，肌萎缩侧索硬化症（ALS）患者的血清降低了神经丝蛋白的水平。无论有无肌肉源性神经营养因子，其作用相似；对运动神经元无特异性。在免疫球蛋白组分中未发现神经毒性活性，也没有证据表明存在可能中和肌肉源性神经营养因子或诱导脊髓神经元细胞溶解的循环抗体。
